FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006216651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41088828
Sequences flagged as poor quality0
Sequence length90
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG464268211.299134645553774No Hit
GTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCACTTGGCGAGG30992357.542768073112234No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATAACCCCATTGCCAAG21753845.294344243646959No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATAACCCCATTGCCAGG9638782.3458396038942753No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATAACCAAATTGCCAAG8406222.045865119345823No Hit
GTTTACCACTACAAAAAAAATAACTAAACAAAAATAACCCCATTGCCAAG7659151.8640468401775783No Hit
GTTTACCACTACACAAAAAAGAACTAAACAAAAATAACCCCATTGCCAAG7200781.7524909690780182No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCCCATTGCCAAG5985701.4567706822886262No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATAACCACATTGCCAAG5630821.3704017062740266No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCCCATTGCCAGG5178051.2602087360583758No Hit
GTTTACCACTACACAAAAAAGAACTAAACAAAAATAACCCCATTGCCAGG4133101.005893864872466No Hit
GTTTACCACTACAAAAAAAATAACTAAACAAAAATAACCAAATTGCCAAG3691020.8983025750941351No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATAACCAAATTGCCAGG3486630.8485591265830216No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCCCATTGCCAGG3454640.8407735552836892No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCCCATTGCCAAG3376710.8218073292331435No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATAACCCCATTGCCAGG3024530.7360954661447145No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATATCCCCATTGCCAAG2915360.7095262001632172No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATATCCCCATTGCCAGG2880540.701051877167195No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATATCCCCATTGCCAGG2828780.6884547789973469No Hit
GTTTACCACTACAAAAAAAATAACTAAACAAAAATAACCCCATTGCCAGG2809810.683837952253104No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCCCATTGCCAGG2730930.6646405198026092No Hit
GTTTACCAATACACAAAAAATAACTAAACAAAAATAACCCCATTGCCAAG2717780.6614401364770005No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATAACCACATTGCCAGG2710620.6596975703468592No Hit
GTTTACCACTACACAAAAAAGAACTAAACAAAAATAACCAAATTGCCAAG2628830.6397919161870472No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATATCCCCATTGCCAGG2612990.6359368536868465No Hit
GTTTACCACTACACAAAAAAGAACTAAACAAAAATAACCACATTGCCAAG2369140.5765898214473287No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCAAATTGCCAAG2328060.5665919699632221No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2228250.5423006954591161No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATATCCCCATTGCCAAG2095800.5100656558030812No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCCCATTGCCAAG1827310.44472185967436206No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCAAATTGCCAGG1747970.4254124746512604No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATATCCCCATTGCCAAG1680540.4090016877580446No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCACATTGCCAGG1670970.4066725874975067No Hit
GTTTACCACTACAAAAAAAATAACTCAACAAAAATAACCCCATTGCCAAG1658950.4037472181002583No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATAACCCCATTGCCAAG1637580.398546290977197No Hit
GTTTACCACTACACAAAAAAGAACTAAACAAAAATAACCACATTGCCAGG1611630.39223070563122414No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCACATTGCCAAG1590960.38720014111865153No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATATCCCCATTGCCAGG1573820.3830286909132575No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCACATTGCCAGG1570950.38233020421025393No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATATCCACATTGCCAGG1447460.35227580596847397No Hit
GTTTACCACTACAAAAAAAATAACTAAACAAAAATAACCAAATTGCCAGG1436730.34966439052484044No Hit
GTTTACCACTACAAAAAAAATAACTAAACAAAAATAACCACATTGCCAAG1419020.3453542164794771No Hit
GTTTACCACTACACAAAAAATACGTCACCAAAAATATCCCCATTGCCAAG1418530.34523496265213505No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATATCCACATTGCCAAG1391980.3387733522114576No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATATCCCCATTGCCAGG1377230.33518356863330345No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATATCCCCATTGCCAGG1346420.3276851800202235No Hit
GTTTACCAATACACAAAAAATAACTAAACAAAAATAACCAAATTGCCAAG1296010.3154166383134608No Hit
GTTTACCACTACACAAAAAAGAACTAAACAAAAATAACCAAATTGCCAGG1274420.3101621686556745No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATATCCACATTGCCAGG1269160.308882015325431No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCACATTGCCAAG1221940.29738984037218097No Hit
GTTTACCACTACACAAAAAATACCTCACCAAAAATATCCCCATTGCCAAG1218730.2966086061155115No Hit
GTTTACCACTACACAAAAAATACCTCACCAAAAATATCCCCATTGCCAGG1217410.2962873509071614No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCAAATTGCCAAG1155750.28128083867468795No Hit
GTTTACCACTACACAAAAAATAACTAACCAAAAATAACCCCATTGCCAGG1140380.2775401624986724No Hit
GTTTACCACTACAAAAAAAAGAACTAAACAAAAATAACCCCATTGCCAAG1092920.2659895775075405No Hit
GTTTACCAATACACAAAAAATAACTAAACAAAAATAACCCCATTGCCAGG1081970.263324619529182No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCCCATTGCCAAG1073470.26125593068753383No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATAACCAAATTGCCAGG1032530.251292151725525No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATATCCCCATTGCCAGG1019590.24814287718306302No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATATCCCCATTGCCAAG1013030.24654633614762633No Hit
GTTTACCAATACAAAAAAAATAACTAAACAAAAATAACCCCATTGCCAAG933830.2272710236466224No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCACATTGCCAGG932550.22695950344458596No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCAAATTGCCAGG909850.22143488736159617No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATATCCACATTGCCAAG907110.22076803942911197No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCAAATTGCCAGG871560.2121160525678659No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATAACCCCATTGCCAAG860150.20933914201690054No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATAACCACATTGCCAGG810590.19727746919430264No Hit
GTTTACCACTACACAAAAAATACCTCACCAAAAATAACCCCATTGCCAGG810360.1972214929079992No Hit
GTTTACCACTACACAAAAAAGAACTAACCAAAAATAACCCCATTGCCAGG786240.19135128410087532No Hit
GTTTACCACTACAAAAAAAATAACTCAACAAAAATAACCCCATTGCCAGG764830.18614062197150036No Hit
GTTTACCACTACACAAAAAATACCTCACCAAAAATAACCCCATTGCCAAG759840.184926179933874No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATATCCACATTGCCAGG755840.18395267930251016No Hit
GTTTACCACTACACAAAAAAGAACTAAACAAAAATATCCCCATTGCCAGG752520.18314467377847818No Hit
GTTTACCAATACACAAAAAAGAACTAAACAAAAATAACCCCATTGCCAAG726260.1767536421335746No Hit
GTTTACCACTACAAAAAAAATAACTCAACAAAAATAACCAAATTGCCAAG726010.17669279834411436No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATATCCACATTGCCAAG725360.17653460449151773No Hit
GTTTACCACTACACAAAAAAGAAGTCACCAAAAATAACCCCATTGCCAGG719280.17505488353184473No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATATCCCCATTGCCAAG718590.17488695467293444No Hit
GTTTCCCAGTCCACTATACTGACGTCTCCAACAAAACAAACAAAAAACAC716780.17444644563724232No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATAACCCCATTGCCAGG713020.1735313550437603No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCAAATTGCCAAG691910.16839370546173768No Hit
GTTTACCACTACACAAAAAATAACTAACCAAAAATATCCCCATTGCCAGG675540.16440965412788117No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATATCCCCATTGCCAAG667840.1625356654125058No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATATCCACATTGCCAGG640840.15596453615079992No Hit
GTTTACCACTACACAAAAAATACGTCACCAAAAATAACCCCATTGCCAGG631930.15379606349443697No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATATCCAAATTGCCAAG631600.15371574969234947No Hit
GTTTACCACTACACAAAAAATAACTAACCAAAAATAACCCCATTGCCAAG620450.15100211668242278No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATATCCAAATTGCCAGG617730.15034013625309536No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATAACCAAATTGCCAAG612970.14918167050177242No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATATCCACATTGCCAGG595870.14501995530269202No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATATCCAAATTGCCAGG574320.13977522065121936No Hit
GTTTACCACTACACAAAAAATACCTCACCAAAAATATCCACATTGCCAGG561290.13660404234455167No Hit
GTTTACCACTACAAAAAAAAGAACTAAACAAAAATAACCCCATTGCCAGG554260.13489311498492973No Hit
GTTTACCACTACAAAAAAAAGAACTAAACAAAAATAACCAAATTGCCAAG544820.13259565349491106No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATATCCACATTGCCAGG544820.13259565349491106No Hit
GTTTACCACTACACAAAAAAGAACTAAACAAAAATATCCCCATTGCCAAG537570.13083118360056412No Hit
GTTTACCAATACACAAAAAATAACTAAACAAAAATAACCACATTGCCAAG537510.13081658109109368No Hit
GTTTACCACTACACAAAAAATACCTAAACAAAAATAACCCCATTGCCAAG521270.1268641685277565No Hit
GTTTACCACTACACAAAAAAGAACTAACCAAAAATATCCCCATTGCCAAG496140.1207481508112132No Hit
GTTTACCAATACAAAAAAAATAACTAAACAAAAATAACCAAATTGCCAAG494170.12026870175026652No Hit
GTTTACCACTACACAAAAAAGAACTAACCAAAAATAACCCCATTGCCAAG478400.1164306755111146No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCACATTGCCAAG475300.11567621252180764No Hit
GTTTACCAATACACAAAAAATAACTAAACAAAAATAACCAAATTGCCAGG474060.11537442732608484No Hit
GTTTACCACTACACAAAAAATACCTCACCAAAAATATCCACATTGCCAAG471140.11466377186518924No Hit
GTTTACCACTACACAAAAAAGAACTAACCAAAAATATCCCCATTGCCAGG470950.11461753058519947No Hit
GTTTACCACTACAAAAAAAATAACTAAACAAAAAAAACCCCATTGCCAAG454100.11051665917557932No Hit
GTTTACCAATACACAAAAAATAACTCAACAAAAATAACCCCATTGCCAAG439030.10684899554691606No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAAAAACCCCATTGCCAAG435610.10601665250709999No Hit
GTTTACCACTACAAAAAAAAGAACTCAACAAAAATAACCCCATTGCCAAG432210.10518917697044074No Hit
GTTTACCACTACACAAAAAAGAAGTCACCAAAAATAACCCCATTGCCAAG429720.10458317282741675No Hit
GTTTACCACTACAAAAAAAATAACTAAACAAAAATAACCACATTGCCAGG421170.10250231522787655No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATATCCAAATTGCCAGG416370.10133411447023995No Hit
GTTTACCACTACACAAAAAATAACTAACCAAAAATAACCAAATTGCCAGG412510.10039468636097383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)