FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006216652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41373405
Sequences flagged as poor quality0
Sequence length28
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTAGCGTGAGCGAACTGCTCGTAGCG4314541.0428293247800127No Hit
ATAGATGCACAGGAATTGACGCTCATGG4170761.0080775319314423No Hit
TACCCGGCATTTGCTCTCATATGGCTCT3999360.966649953031422No Hit
TATGACTCAATGCGCTAGGACGGAGGTT3916570.9466395139583025No Hit
TAGAGCCGTACGGTACTTGATGCTCCAC3861120.9332371846117089No Hit
TGATGACTCGTTTCGCCCTAACATGCTT3856860.9322075376682195No Hit
GTCCATTGTCTTTGACATAATCTATTAT3854940.931743471440168No Hit
GCCCTCATCTCGCCTGTGTGGTATTAGG3762340.9093619439830972No Hit
CTCATGCTCCTGTTCAGCTTCTATCATA3707600.8961312224604187No Hit
GAAGGCCCAAGCCACTTTGCTGATAATA3641910.8802538732308836No Hit
ATCGAGGCAATTAGCTATGTGATCGTAT3541440.8559701576411224No Hit
GGCTTAAGTTATCCTACACATCAGGGTA3447440.8332502485594309No Hit
GCACTTACACAAGCCTGGGTCATTTATG3447110.8331704871764845No Hit
GCCAACATCAGCTAACGATTTCAGAAAA3437560.8308622410942488No Hit
CAGCCTTTCCAACTGGCATTACGCAGGC3437310.8308018158041379No Hit
GAGGCTACAAGTGAACACCTAGCGTTCG3433670.8299220235801236No Hit
CGACCTGCAGGCTGTTCTCATTCTTCAT3386130.8184315504126383No Hit
AGATGAAGTAACCAGCTGTAATGCTCAC3363840.8130440315463521No Hit
AGCGCCTAGTCATGCGGCCCTCGTTGGT3343450.8081157448849084No Hit
GGAACGGTCCCTCAACGGATCATGCATG3221520.7786451223920293No Hit
AGAAAGGCAGGCAAGCTCTGTCAACGTC3215620.7772190855454126No Hit
AATAACCGTCATGCCCGAGTAATTGCTA3193150.7717880604702465No Hit
TCCGCCATCCACCTGTTACTTACGCATT3183330.7694145550746911No Hit
GGTCAGGAGCACTAACTTCCTTACGACA3179600.7685130097462367No Hit
TGCAAGCGTACGTTTCCTTTCCCCCGAG3174810.7673552611877122No Hit
GGCAATCGTTTGTGGAATCTCTCCGGTG3155730.7627436030464497No Hit
GCTCACAAGCCTAATATTATTTTCTTAG3073300.7428201763910899No Hit
ACCGGTTCAACCCTCCCGAAAATACACA3066880.7412684549410424No Hit
GTAGCCATCCTTAGTTCGCAGTTTCTGG3047080.7364827719642606No Hit
ACTCGCTTCATGGCCAAGTTTAATGTAC3036870.7340150031161322No Hit
TTGCCCGTCTGTGAGTTAACGGATATGA3016760.7291543927796129No Hit
TGTGCGGGTTCCTCCTGTCGTGCCGGCT2983220.721047735858337No Hit
CAACCCGCATTGTGATCACCTGGGTGAA2941760.7110268057463484No Hit
TAATCACCAGGTTTGCGTACCATAGTAT2910660.7035098996565547No Hit
GGAACGGTTTTTTTTTTTTTTTTTTTTT2906000.7023835722488878No Hit
TGCTTTAGTGCTGTAAACGACAATCGGC2862070.6917656402706038No Hit
TTTCACCCAGAAACGTGTGGCTACTTTT2856440.6904048627373067No Hit
TTAAGCCAGGAACCGGAGTGTAGGTGGA2835840.68542581883217No Hit
ATATGCATCCTAACGGGTCCATGACAGT2803160.6775270249088756No Hit
AAGCATGAGTTATCCTAAACACACTTCG2738200.6618261175264641No Hit
AGTGCCGGTATTGAGTTGTAACGTATGT2735690.6612194476137508No Hit
TAGCGGCTCGCTAGCAATACTCTAATGG2722390.658004822179852No Hit
ACCACATAGGTGCTGCTTCCCATAGCTG2696590.6517689322404091No Hit
GAGCTTAGTTGCCTCACGAGAAATGGTC2683400.6485808939341589No Hit
CTTTGGGTTTTTGTTTTAAATTCCTTTT2683100.6485083835860259No Hit
TAAGCTATCAATTACGTGTTTATCAGAA2677840.647237035482093No Hit
AACGACAAGTAACTCATGTACCACCACT2666920.6445976588100496No Hit
AGGTGAGGTTGTAAACAATGGCCTGGTT2662260.6434713314023828No Hit
CGATCCTTCCAGCACACTCAAACGAGCA2616880.6325029327414555No Hit
GAAGCTAAGTAGAGGCATATTAGGGATG2615490.632166968128439No Hit
GCTATCCTCCTGGCTTCTTTCGGTGGTT2609320.6306756719685025No Hit
GAACCTGTCAAGCTACCCGGGCGGATCA2608120.6303856305759702No Hit
TCTTGACGTAACGGGAGAAAATAGTAGT2596810.627651990451354No Hit
CCAGTTTGTATGGTGCCAAGCCCATGGC2570970.6214064324654932No Hit
GTATTGCAGGACAACAGGAATCTACTGA2540790.6141118914433076No Hit
CCAGCTGCACTTGTTCTGAACAAAAAAT2533360.6123160518212123No Hit
TCCACAACATAGACTTCTGATATCGACC2525900.6105129611643034No Hit
ACATTAGTCACGCGGTTCGTGTCCACTT2488930.6015772692627064No Hit
GTTGCTGTCAGGAAGATAGGCACCGCTT2439560.5896444829716094No Hit
ACTCAGTAGCATTTCTACCCAGTTTTCT2418450.5845421714746466No Hit
GTTACTAAGCTTATGAAAGATCATAAAA2412510.583106466581612No Hit
ATTTGTGAGCGGCTGTATGTTTTCTTTG2379770.575193170588691No Hit
GCTAGTGTCGAGGTGGCTAAAACCATCG2374800.5739919158212867No Hit
GTGCTTACACCACAACTATCTTAGTTAA2349940.5679832249726606No Hit
ATTGCGCCACAACAGGCACGTCAAAGAT2333910.5641087553707508No Hit
TCGATTAAGCTGGCTAGACGATACGTTT2318370.5603527193374584No Hit
GCCTTAACACGTGCTGAATTGCTACCAT2308380.5579381247446277No Hit
TCCCGGACACGTAAGGAATTTGCTGAAT2270940.548888833297622No Hit
CGGAGTCTCAATAGCCTCATAGTCTTAG2264720.5473854520796633No Hit
CCCAACCGTTAGTGATGTTATTTCCATG2264300.547283937592277No Hit
TGAGTGAAGCGAGTAAATGGAATTATTG2251390.5441635756109511No Hit
TGATGATGTGATTACGAGATCCTGATAG2248910.5435641567330511No Hit
TCTAAGGGTGCACGCATGGGAAATATCA2231400.5393319694136849No Hit
GTAGTTTCATAATCCGCGCCCAAATATA2225230.5378406732537484No Hit
CCTATATTCACTAGGTGTATCTAAATGT2193190.5300965680731378No Hit
AATTGCTCATGTCAATGACTAGCCAGTA2178910.5266450755020042No Hit
GTTCATTTCACACAGTGTCGTTGACAGT2171830.5249338312860641No Hit
GCGGGTTTCACAGACTGGCCATTCGAGT2164760.5232250040817283No Hit
TCATGTTTCCGCAAATTCATGGTACGCT2162050.5225699939369264No Hit
GTGATGAAGTTTGAGCCTTGTACTACAT2139810.5171945601286624No Hit
AAGTTAGCAGGTTCACTTTCTAAAGGTT2124610.5135207024899208No Hit
AAAGCCGCAATTGCGCACCTAATTTGCA2077070.5020302293224356No Hit
TCCTGGTTCTTGAGCGTGAATCTAAATA2040400.4931670477689714No Hit
ACACTAATCCGGAACCAGCATCACTGTA2028820.49036814833103537No Hit
GCGAAGTAGTTCCTGCAATGCATTTTAC2013600.48668945666908486No Hit
CCATCACTCTTTAGGAGCCGTCGGCGCA1995270.4822590743981551No Hit
TTGTAAGTCGAGCAAATTGAGTTCTAAA1992520.48159439620693534No Hit
ATGGTTATCAGCACCACCGGACTTCGAG1984130.4795665234708142No Hit
AGACACGCAAACGGGCCTCTTCCACCAT1933970.4674427932629669No Hit
TGGGCCTAGCATGTTAGTTAAATGTACC1933260.467271185439052No Hit
GCCTGTGCAATGAAGCAAGGCCCTTATA1925280.46534241017871264No Hit
CTGTGTGAGGATTGCTACCTCTCGTCGT1902890.4599307211963821No Hit
TGATTCAAGCGGATTTGAGTCGATCTAT1901310.45954883336288127No Hit
GTGAGGAGTAAACAAGGACCCTATGTGT1885020.45561152145925626No Hit
GGTTTCCTCAGCTAACGAGGCCACAATA1880890.4546132956666245No Hit
GCTTACCTCACTTTACGATCCTTCTTCA1870620.4521310247488695No Hit
CATAGCTAGTTATCTCACGTACAGTCTT1858680.44924511289317376No Hit
AGTAACGAGGTACCGCGCTTTTGGCTGC1848330.44674350588258327No Hit
GGGCATGAGCCTGTTCGACCAGCATACA1820190.4399420352277025No Hit
GTCTAATCAAATATCCTAGGTTCCCCCC1787280.43198765003750594No Hit
TCACATAAGTTGTCCCTTGCTGAATTTT1780970.4304625157151073No Hit
CGTTAAATCTGTGCCTGTTAGTACTAGG1775140.4290533979497216No Hit
GGGTGAAGTAGGTGTCGGAAGGTGGCGC1762990.4261167288503327No Hit
TGAGGCACATAAGTCTCCTTTTTTGTTT1735130.4193829345203761No Hit
AATCTCAAGAGAGGCTGCATGTATAGGA1731880.4185974057489346No Hit
CGACTAAGTCACGGATATATCTAAACTT1730910.41836295562330444No Hit
GGCATTAGTTGTTGCTGGGTAAAACTGA1707400.4126805613412771No Hit
CCTTACTCAATCCCTTTTACCACAGCCT1695770.40986957684531883No Hit
GAATTTGTCCATAAGCTTCATCCCTAAT1674030.4046149936172766No Hit
TATCCAGCATCAGCACAGTTTGTTTAAA1672270.40418959957489603No Hit
CTAATTGAGGCGAAACCTTTTTTGTTTT1662570.4018450983185938No Hit
TACTGCACATTGCAGCTCAGGACTCATC1661210.40151638474039064No Hit
TTGAGCTAGTTCCCACTGTCATTTCTGG1656860.4004649846924613No Hit
TAGGTTGGTACCTTACGCAGGGTGAAAG1630910.39419283957895174No Hit
GTCTAATCAAATATCCAGACTGATTGAG1627190.39329371126210183No Hit
AGTAACCTCTGTGCAGATTTTACTCTAT1607340.3884959432272978No Hit
CTTTCTTGTTCAAGATGTTGCAGGCACG1605080.38794969860469547No Hit
TCTAGCACAGCAGGTAAAAACTTTACAG1601440.387069906380681No Hit
CAAAGTGAGTTAGGCTTTTGCTTCTGGC1586060.38335254253305956No Hit
TGTTGTAAGCTTAACAAGAATCAGGATC1552360.37520721342611274No Hit
GAAGCCTGTTAGCTGACTTTAGTAATTA1509650.36488415686356973No Hit
TTTGTCCCAGTAGGTGTTTGTAATAGTC1507190.3642895720088786No Hit
GAGCATGCAGGTCCTGATTCCGGTAGGA1495890.3615583488958668No Hit
CGAAGGCCAAGCCACTTAATTTCGTCAG1486270.3592331837324001No Hit
GAGCTTGTCCAATAGCTGTTCAGGGTTA1461970.3533598455336224No Hit
TGCCGGTAGCCTTAAACGTTCACGCCTC1453180.3512352923333238No Hit
ACTCAGTAGCATTTCTGTAATTACACTG1435360.3469281776542201No Hit
TGTTACTTCAATCTAGTACACGACTCAT1392350.33653261074354407No Hit
AATGTCATCACTTTACATAGAACAGGTG1370610.33127802751550184No Hit
GCCACTAAGTTGGCCATCGATATTGTCA1356820.3279449685129856No Hit
TGTGGCGGTAGTAAGACTTTTAACATGT1330300.3215350537380232No Hit
TGAGCCGGTGAATCGCGGCTTCGTCCGG1312220.31716509675720433No Hit
TTGTCCGGTGCTGGTGTTTTCTCGGCGG1296800.3134380648631651No Hit
AGTGCACGTCTTTATGAGGCAGCACAGC1256670.3037385972945664No Hit
AGGCAGGTCCTGGCTTCGATATTCTATT1252230.30266544414219715No Hit
GTTAGACTCCAAACACTGGATTTCCGTT1250300.3021989609025411No Hit
GGTTGAGCAGCACGTTCTCTTTGCTAAT1233460.29812871336067215No Hit
GAGGTACAGGTTACACGGCATGCTGATC1200160.2900800647179027No Hit
GGCTAGACACCGTTCCTGCCGTTATCGG1159490.2802500785226645No Hit
CTCTCACAGTGCACGCACGGTTAGGTCG1131070.2733809315428595No Hit
TTGGATCAGTGAACAGCCAGTAACTGGT1082450.2616294211220952No Hit
AGCATCCCAATATACCTAGGGAATTTGT1066150.2576896922068658No Hit
GATTATGTCTCATCATCGAATACGTCTG1066140.2576872751952613No Hit
CCTGTAACATCCCTCATGTCCATTAGTA1059300.2560340392578276No Hit
AGCCTCCGTCCTTCAGATCATGGAGTTG1037730.2508205452270607No Hit
CCTTCAATCGGTCATGAGGCGTAAAGTG1031680.2493582532063774No Hit
TTGGATATCCGGTTAGTCCTGCCTCCAT1030320.24902953962817417No Hit
TCAAGTATCTTACTCGCTTTGAACAGAC1007770.24357917846017266No Hit
TGTGCGGGTGCAATATAAAATAGTAATG982890.23756565358833776No Hit
ACTAAAGCAACTCGCGTATTATTATCTT967960.23395705526291585No Hit
CTTACCGGTGGGAACAGACAATGTTAAA950230.22967169368825216No Hit
GCGCAAACAGGCCTTGCGCGCTAGAATT933270.22557244200712995No Hit
AAGTTAGCAACTAGAACGTAAACCAGTT881190.21298464557123106No Hit
CCTACTTCATGTCAGCCCTCGTTCAGCT853000.20617108985832808No Hit
AGGAACCAGCACAGAACATTTCATTTAC844160.2040344516000073No Hit
CGATCCTTCACAGACTTTTGCACATAGT843570.20389184791534562No Hit
CATCACACAAGTAAGCATGTTATACAGT836890.20227728416358287No Hit
CGGGCTTAGGGATGCGACAATATCTAAA834780.20176729471504704No Hit
CTACCTCAGTAGAGGCTATGCTTTAAGT832200.2011437057211027No Hit
TGTATCGCAGGTATTTCATCATATTCCT830800.2008053240964818No Hit
ACCCAGGGTACAATGTTTTCCAGACTCT798710.19304913385784903No Hit
CTTATGTTCACTCGCTAGTTGTGACGGG764610.18480712428672477No Hit
TACTAAGTCATCCTGCAAAGTCAGAATT757580.18310796512880678No Hit
TGTGATCAGGTCCAATCATCAACTAGAT742880.17955495807028696No Hit
CTTGTAAAGTTAGTGCGTCATATAATTG739350.1787017529739213No Hit
GTTGCGGGTTAAGTGTGAGTAGCAGGGG728050.17597052986090944No Hit
CGATCCTTCTCCATATGTCAACGCCCGA719670.17394507413639268No Hit
CATGGATTCATAGACCTACTTTCGCAGC716870.17326831088715083No Hit
TAGGTGCGTGTGAGGATAAGTTTTGTGG716120.17308703501681819No Hit
GTCTATGCATGACTATAACTTCCAAGCC714120.17260363269593113No Hit
GTGGCTTCATACCCCGGAAACTAGTGTG703000.1699159157917991No Hit
GCCAGGTTCCAGGTCACCCAAGAACAGT699470.16906271069543347No Hit
TCGTAATCAATTAAGGTCTACGCTGGGC694810.16793638328776664No Hit
TGTTGCACAATTAGCTACGATAAAGGGA691540.16714602049311628No Hit
ACGTTGTAGGCCTAATATTCTTACATTA639200.15449538175550212No Hit
CTGTTTAGTGTTAAACTCTGAGTCCGAT626450.15141369195984716No Hit
AACGGTAAGTCCTGAGCGCTATCTACGA620820.1500529144265501No Hit
ACTTGTCGTTACTTGCTATATTTAGGGC603780.1459343266525924No Hit
GTGCAAGCATGCTCCCACGTATTGTATT600160.14505936845178685No Hit
CGTTAACAGCTGCACATTTCTCAGTACG592250.14314751227267855No Hit
TTCAACCGTTTATCTGACACTTTATGAG580600.14033169375351146No Hit
CTAATCTTCAATAGCCATCCGCTCCTGC571420.13811287710063988No Hit
TTTAGCTTCCAAGTTACGTCCGTCACAG562380.1359278986102304No Hit
GGAAACTCACTTAGGCAAACTAAATTCC553550.13379367736351408No Hit
TTACCTGTCGGTTCCTTTCAGTGCCTTG551400.1332740198685605No Hit
AAAGCACCACCTGGTGATACATCTTCTT534300.1291409300249762No Hit
CTGATCACACGAATCCCTACTCAGCGTG527780.1275650384588844No Hit
ACACTTAAGTACTGGTCTATGCGGGAAT516380.1248096452298282No Hit
GAGGACTAGCTAGAAGTCGCTAGATGTG513810.12418847324748833No Hit
TCTATGTTCCCGCCTAACATCCAGTCAA510550.12340052746444244No Hit
TTCAGCACAGGTCCTGAGAAATTTCTAG499540.12073939768795922No Hit
CGCAAATTCTTAATGGACAATTCTACAC491350.11875986518392673No Hit
GCTGGATGTCGTAAATTATGGCTCTCTT485020.1172298968383192No Hit
TATTCGTTCAAATCGCACAGCTAATATT480110.1160431441405415No Hit
GCGAGTGAGCACTAACTTCTATCCCATG471070.11385816565013201No Hit
CATTATCTCCACCTGTTGCCTTAGGAGT467100.11289861204317121No Hit
ATTTGCGCAGGCTAGAGGCATATGTTGG459120.11096983678283187No Hit
AGGATTGAGGAGTCTTAAATTAGTGAGG455030.10998127903661785No Hit
TACTCAAAGTCACCTCACTTTTAGATGT452950.1094785406228953No Hit
TTCGCAACAAACTCATTGTGATATACAC449680.10868817782824498No Hit
GCTTTCATCCGGCTAACTTCAGAGGACT446200.10784705778990152No Hit
GACACCGGTTATCGAGCTGTACGGGCTT438070.10588202735549562No Hit
GTCTAGCCAAGGACCATCACGTTGAGGA428490.10356653023844666No Hit
GGAATCTTCCTCCATAAAATCGGTGTAT419320.10135013059717952No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)