FastQCFastQC Report
Thu 7 Jul 2022
EGAF00006216653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41373405
Sequences flagged as poor quality0
Sequence length90
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG468746911.329666968430566No Hit
GTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCACTTGGCGAGG31384847.585752248334407No Hit
GTTTACCACTACAAAAAAAATAACTAAACAAAAATAACCCCATTGCCAAG15548253.7580300678660605No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATAACCCCATTGCCAAG11105172.684132475922637No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATAACCCCATTGCCAGG9731362.3520810046937157No Hit
GTTTACCACTACAAAAAAAATAACTAAACAAAAATAACCAAATTGCCAAG7750901.873401524481729No Hit
GTTTACCACTACACAAAAAAGAACTAAACAAAAATAACCCCATTGCCAGG6060091.4647307853922102No Hit
GTTTACCACTACACAAAAAAGAACTAAACAAAAATAACCCCATTGCCAAG5347201.2924244451236249No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATAACCCCATTGCCAGG5255501.2702604487109532No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATAACCAAATTGCCAAG4664781.1274827392137534No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCCCATTGCCAGG4264041.0306234161776144No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATAACCAAATTGCCAGG4018020.9711600966852982No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCCCATTGCCAGG3769660.911131196477544No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCCCATTGCCAAG3637470.8791807200785142No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCCCATTGCCAGG3262520.788554869970214No Hit
GTTTACCACTACAAAAAAAATAACTAAACAAAAAAAACCCCATTGCCAAG3202010.7739295327517761No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATAACCCCATTGCCAGG3020110.7299640916670987No Hit
GTTTACCACTACACAAAAAATACCTCACCAAAAATAACCCCATTGCCAGG2895280.6997925358089333No Hit
GTTTACCACTACAAAAAAAATAACTAAACAAAAATAACCACATTGCCAAG2624730.6344002868509373No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCCCATTGCCAAG2537520.6133215286486572No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATAACCACATTGCCAAG2422750.5855814864645538No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATATCCCCATTGCCAAG2378090.5747871126391458No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATAACCCCATTGCCAAG2313520.5591804687093073No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATAACCACATTGCCAGG2286040.5525385208203193No Hit
GTTTACCACTACACAAAAAAGAACTAAACAAAAATAACCAAATTGCCAAG2260940.5464718216931866No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2223130.537333100816817No Hit
GTTTACCACTACACAAAAAAGAACTAAACAAAAATAACCAAATTGCCAGG2205560.5330864114278242No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATATCCCCATTGCCAAG2149920.5196381588607464No Hit
GTTTACCACTACAAAAAAAAGAACTAAACAAAAATAACCCCATTGCCAAG2025880.4896575469193314No Hit
GTTTACCACTACACAAAAAAGAACTAAACAAAAATAACCACATTGCCAGG2022350.4888043418229657No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCCCATTGCCAAG1915100.4628818923653975No Hit
GTTTACCAATACAAAAAAAATAACTAAACAAAAATAACCCCATTGCCAAG1809700.4374065900546498No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATATCCCCATTGCCAGG1724260.416755642906355No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCAAATTGCCAGG1661470.40157922704210597No Hit
GTTTACCACTACACAAAAAAGAACTAACCAAAAATAACCCCATTGCCAGG1604420.3877901758388027No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCAAATTGCCAAG1491580.3605166168943552No Hit
GTTTACCACTACAAAAAAAATAACTAAACAAAAAAAACCAAATTGCCAAG1443190.34882069774049296No Hit
GTTTACCACTACACAAAAAAGAACTAAACAAAAATAACCACATTGCCAAG1438630.3477185404488705No Hit
GTTTACCACTACACAAAAAATAACTAACCAAAAATAACCCCATTGCCAGG1318330.3186418908475142No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCACATTGCCAGG1273750.30786685311494183No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATAACCCCATTGCCAAG1233050.2980296158848903No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCACATTGCCAGG1197000.2893162890509012No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCAAATTGCCAGG1174350.2838417577668553No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCAAATTGCCAGG1108680.26796924256052895No Hit
GTTTACCACTACACAAAAAATACCTCACCAAAAATAACCCCATTGCCAAG1104790.2670290250464036No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATAACCAAATTGCCAGG1073370.259434774585268No Hit
GTTTACCACTACACAAAAAATACCTCACCAAAAATAACCACATTGCCAGG1048090.2533245692492556No Hit
GTTTACCACTACAAAAAAAAGAACTAAACAAAAATAACCAAATTGCCAAG1029460.24882167663019275No Hit
GTTTACCACTACACAAAAAAGACCTAAACAAAAATAACCCCATTGCCAGG1011440.2444662217190004No Hit
GTTTACCACTACAAAAAAAATAACTCAACAAAAATAACCCCATTGCCAAG1010880.24433086906915205No Hit
GTTTACCAATACAAAAAAAATAACTAAACAAAAATAACCAAATTGCCAAG1006660.24331089017208032No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCAAATTGCCAAG990360.23937116125685087No Hit
GTTTACCAATACACAAAAAATAACTAAACAAAAATAACCCCATTGCCAAG960830.23223372598895356No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATAACCAAATTGCCAAG929560.22467573070188446No Hit
GTTTACCACTACACAAAAAATAACTAACCAAAAATAACCCCATTGCCAAG890080.21513336888757403No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCACATTGCCAAG876510.21185348414035537No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATAACCACATTGCCAGG830840.20081499214289955No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCACATTGCCAAG825880.19961615438709962No Hit
GTTTACCACTACACAAAAAAGACCTAACCAAAAATAACCCCATTGCCAGG793850.19187446621809348No Hit
GTTTCCCAGTCCACTATACTGACGTCTCCAACAAAACAAACAAAAAACAC761570.18407235275897646No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATATCCACATTGCCAAG755840.18268740510963505No Hit
GTTTACCACTACACAAAAAAGAACTAACCAAAAATAACCCCATTGCCAAG735180.1776938591348718No Hit
GTTTACCACTACACAAAAAAGACCTCAACAAAAATAACCCCATTGCCAGG679550.1642480235793984No Hit
GTTTACCACTACACAAAAAATACCTAAACAAAAATAACCCCATTGCCAGG663270.1603131286873778No Hit
GTTTACCACTACACAAAAAAGAAGTCACCAAAAATAACCCCATTGCCAGG649010.1568664701394531No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCACATTGCCAAG648130.1566537731182628No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCAAATTGCCAAG637480.15407965575953925No Hit
GTTTACCAATACAAAAAAAATAACTAAACAAAAAAAACCCCATTGCCAAG628120.15181733289778784No Hit
GTTTACCACTACACAAAAAATACCTCAACAAAAATAACCCCATTGCCAGG601920.14548476249416745No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATATCCACATTGCCAAG599220.14483216936096993No Hit
GTTTACCACTACACAAAAAATACCTCAACAAAAATAACCCCATTGCCAAG569840.13773098926713911No Hit
GTTTACCACTACACAAAAAAGAACTAACCAAAAATAACCACATTGCCAGG543040.13125339816725262No Hit
GTTTACCACTACACAAAAAATAAGTCACCAAAAATAACCCCATTGCCAGG540330.13059838802245066No Hit
GTTTACCACTACACAAAAAAGAACTAACCAAAAATAACCAAATTGCCAGG515790.12466704154516652No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATAACCACATTGCCAAG507430.12264641984385863No Hit
GTTTACCACTACACAAAAAAGACCTAAACAAAAATAACCACATTGCCAGG503820.1217738786546575No Hit
GTTTACCACTACACAAAAAATACCTAAACAAAAATAACCCCATTGCCAAG503590.1217182873877555No Hit
GTTTACCACTACAAAAAAAATAACTAAACAAAAAAAACCACATTGCCAAG503510.12169895129492002No Hit
GTTTACCAATACACAAAAAATAACTAAACAAAAATAACCAAATTGCCAAG488680.1181145230855425No Hit
GTTTACCACTACAAAAAAAATAACTCAACAAAAATAACCAAATTGCCAAG476530.11517785398615366No Hit
GTTTACCACTACACAAAAAATAACTAACCAAAAATAACCAAATTGCCAGG466930.11285752284589581No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATATCCCCATTGCCAAG462720.11183996096042856No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATAACCACATTGCCAAG460340.11126471219857297No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAAAAACCCCATTGCCAAG452420.10935043900786023No Hit
GTTTACCACTACACAAAAAAGACCTAAACAAAAATAACCCCATTGCCAAG451260.10907006566174575No Hit
GTTTACCACTACACAAAAAAGACCTCAACAAAAATAACCCCATTGCCAAG449450.10863258656134296No Hit
GTTTACCACTACAAAAAAAAGAACTAAACAAAAATAACCACATTGCCAAG443900.10729114512088139No Hit
GGTTACCACTACAAAAAAAATAACTAAACAAAAATAACCCCATTGCCAAG439690.10627358323541415No Hit
GTTTACCACTACACAAAAAAGACCTAACCAAAAATAACCCCATTGCCAAG426360.10305170676670194No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATATCCAAATTGCCAAG416630.10069995447558643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)