FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006219717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006219717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74768817
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACT1310650.17529366553973966No Hit
AACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCT1261710.16874815606618465No Hit
CTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCT1232300.16481469808463067No Hit
AAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAG1214770.16247013778484684No Hit
TATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTAC1203920.16101899806706851No Hit
GGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATA1172040.15675518846312628No Hit
ATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACC1166540.15601958768452898No Hit
TAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGA1056660.14132362158411574No Hit
TTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCT1030130.13777535091935453No Hit
AGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGAC1027420.13741290035390022No Hit
TAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTC1027220.13738615123467848No Hit
AGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCC1027020.13735940211545675No Hit
AAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTC1013850.1355979726147065No Hit
CAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCC999210.13363993708767652No Hit
ATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGC993460.13287089991005208No Hit
CAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCA993220.13283880096698603No Hit
AAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAG991580.1326194581893679No Hit
CTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTA977310.13071090853289813No Hit
ATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCT957590.1280734453776365No Hit
TCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCT940910.125842568834545No Hit
TTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTC923330.12349132125495578No Hit
AATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGG921690.12327197847733769No Hit
CTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTA918410.12283329292210148No Hit
GCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCT917620.12272763390117568No Hit
CCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTC916550.12258452611333948No Hit
ACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTT915640.12246281762088064No Hit
TGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTC913050.12211641652695937No Hit
AAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGG905090.12105180158193489No Hit
TAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCG893360.11948296573958098No Hit
TTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGG892170.11932380848021175No Hit
AGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGC891930.11929170953714567No Hit
TTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTC889940.11902555580088957No Hit
CAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATT886820.11860826954103072No Hit
AGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGAC879130.11757976590695558No Hit
TCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTT872950.1167532181230044No Hit
ATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTT871640.11657801139210214No Hit
TCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGC870130.11637605554197816No Hit
AAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCT868790.11619683644319262No Hit
AAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCG867710.11605239119939534No Hit
TAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAA867570.11603366681594011No Hit
CTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGT867280.11599488059306864No Hit
TGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATC865840.11580228693467225No Hit
TACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTT860470.11508407308356905No Hit
TTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAG852570.1140274828743111No Hit
TTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTG848120.1134323149716278No Hit
AACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTT838100.11209218409861962No Hit
CGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGC834830.11165483599934448No Hit
GTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTAT832270.11131244727330646No Hit
TGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTA830560.11108374230396076No Hit
CTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGC830090.11102088187378971No Hit
CAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTA829140.11089382355748655No Hit
TCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCT824660.11029464328692No Hit
TAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGA814650.10895584986987288No Hit
CTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCT809070.10820954944358689No Hit
AGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGC806560.1078738479973543No Hit
CTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTA804760.10763310592435882No Hit
TATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCT801990.10726263062313798No Hit
CAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCC799850.10697641504746556No Hit
ATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTA796480.10652569238857959No Hit
TACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGA792200.10595326123723477No Hit
CAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTG787970.10538751736569538No Hit
CAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATC787880.1053754802620456No Hit
ACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTT785660.10507856503868451No Hit
TGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATA781110.10447002257639036No Hit
TATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTC777430.10397783878271072No Hit
TGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGT776010.10378792003623649No Hit
ATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTG761490.1018459339807396No Hit
GGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCA753840.10082278017050877No Hit
CGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACAT753570.10078666885955946No Hit
TTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGG748750.10014201508631598No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)