FastQCFastQC Report
Thu 7 Jul 2022
EGAF00006219724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006219724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70453500
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC1312910.1863512813415941No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT1300010.18452028643005672No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG1257660.17850922949179246No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT1214790.17242436500670655No Hit
AAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAG1184530.16812933353204595No Hit
TACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACA1155240.16397198151972578No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA1046730.14857033362430538No Hit
AAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTT1043970.14817858587579041No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTG1036700.14714669959618756No Hit
TCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGA1036410.14710553769507548No Hit
TCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAA1003060.14237191906718616No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG987430.14015343453483503No Hit
CTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAAC986250.1399859481785859No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTG985010.1398099455669342No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA983790.13963678170708338No Hit
TCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACT960730.13636370088072275No Hit
TAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAA938690.1332353963962046No Hit
TGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTC914900.12985870112911352No Hit
CAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTA914680.12982747485930435No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGAC909840.12914049692350274No Hit
AAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTC896520.12724988822414784No Hit
ACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAG885310.12565876783978083No Hit
GATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGCACAA859670.12201948803111272No Hit
CAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGT848910.12049223956226446No Hit
TACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTT847930.12035314072402364No Hit
TATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCC836550.11873789094934957No Hit
AAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGA831930.1180821392833571No Hit
CTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTC815740.11578416970058265No Hit
TTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTT811820.11522777434761935No Hit
TAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGA806750.11450815076610814No Hit
ACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTG805010.11426117935943567No Hit
TTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTT803690.11407382174058067No Hit
ATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCA798860.11338826318067945No Hit
CTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTA797190.11315122740530989No Hit
CTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAG794670.11279354467840491No Hit
TGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAG787650.11179714279631245No Hit
CTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGG786000.11156294577274373No Hit
TGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAA779510.11064177081337337No Hit
CTTTGTGTTTGAGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT777970.1104231869247092No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGC770590.10937568751020177No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG762110.10817205674664851No Hit
TTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTC759630.10782005152334519No Hit
CTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGT759390.10778598650173518No Hit
CAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATT755220.10719410675126147No Hit
ACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTT755050.10716997736095438No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT754260.10705784666482149No Hit
TGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAA752910.10686623091826523No Hit
TTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCC750490.10652274195036443No Hit
AGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGG750230.1064858381769536No Hit
TTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCT748450.10623318926667943No Hit
TTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCT741260.10521265799427991No Hit
ACTAGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACT734420.10424180487839498No Hit
AGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGAC727770.10329791990461794No Hit
TGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATC724800.10287636526219422No Hit
ATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTC723780.10273158892035172No Hit
TACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGA720960.10233132491643425No Hit
TTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTAC717780.10187996338010177No Hit
AACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAA716550.10170538014435053No Hit
AAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAG715240.10151944190139595No Hit
CTTTGTGTTTGATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTT712030.10106382223736222No Hit
CCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTT707850.10047052311098811No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)