FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006219753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006219753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89275543
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCT1327750.14872494250749052No Hit
AACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCT1281770.14357459578823284No Hit
TATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACT1276700.14300669109343866No Hit
TATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTAC1240760.1389809524877379No Hit
AAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAG1235910.13843769060021285No Hit
ATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACC1222370.13692103782555543No Hit
AAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCT1218980.1365413145680895No Hit
TAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGA1148540.12865113573154072No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA1124430.1259505080803597No Hit
CAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCC1106030.1238894732905741No Hit
TAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTC1102730.12351983118153646No Hit
TTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCT1084690.12149912098546407No Hit
AGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGAC1074710.12038123363752602No Hit
TACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT1074530.12036107134066941No Hit
AAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTC1067340.11955569959400862No Hit
CTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTA1062600.1190247591101182No Hit
GGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATA1056120.11829891642328068No Hit
TTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCT1039280.1164126215395856No Hit
TATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTC1035990.1160440995581511No Hit
GCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCT1034630.11589176220412346No Hit
CAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCC1032340.11563525298300342No Hit
CCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTC1030760.11545827282170662No Hit
TTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGG1018220.11405363280736361No Hit
ATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGC1015710.11377248077897437No Hit
AGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCC1006660.11275876529812873No Hit
TCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTT1003690.11242608739999488No Hit
TACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTT1002740.11231967527769615No Hit
AAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAG1000430.11206092580136981No Hit
TCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGG999420.11194779291345223No Hit
CTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGC999130.11191530921296104No Hit
TCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCT993570.11129251826561279No Hit
CAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCA992910.11121858984380525No Hit
TATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTA987770.11064284425578907No Hit
TATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCT987670.11063164297975762No Hit
GGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTC987220.11058123723761612No Hit
TAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCG976870.10942190516836173No Hit
TTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTC974460.10915195441600395No Hit
CAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATT973220.10901305859321404No Hit
ATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCT968080.10843731300519785No Hit
CTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTA965440.10814159931796774No Hit
AGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTT960950.10763866202415594No Hit
TCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGC959790.10750872722219118No Hit
AAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGG959360.10746056173525599No Hit
TTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTC956770.1071704486860416No Hit
ATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCG956760.10716932855843846No Hit
TTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTG956400.10712900396472526No Hit
AGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGG955550.107033793118458No Hit
ATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTT946560.10602679840323122No Hit
ATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTG946390.10600775623397776No Hit
CGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGC940930.10539616656266096No Hit
TTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAG939950.1052863940575528No Hit
TGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTA939470.10523262793260188No Hit
AATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGG938880.10516654040401635No Hit
TAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAA933180.10452806767022409No Hit
TGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTC931940.10438917184743418No Hit
AAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCG927110.10384815021511545No Hit
CTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGT924980.10360956303564572No Hit
TCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTT924660.10357371895234511No Hit
TACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGA911470.10209627064379771No Hit
CTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT909170.1018386412950745No Hit
TCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCT907310.10163029756088966No Hit
AAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTT896410.10040935847346233No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)