FastQCFastQC Report
Thu 7 Jul 2022
EGAF00006219775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006219775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50099739
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT6830171.3633144875265717TruSeq Adapter, Index 6 (97% over 36bp)
AAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAG1141450.2278355182648756No Hit
AAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTC1023620.20431643366445482No Hit
AGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGAC992660.19813676075238637No Hit
AAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAG986960.19699903027438925No Hit
TAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGA969200.1934541016271562No Hit
CAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCA926590.18494906729953223No Hit
TGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTC901700.17998097754561157No Hit
TTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCT891010.1778472338947714No Hit
ATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGC889530.17755182317416865No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT871940.17404082683943722No Hit
TAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAA864960.1726476060084864No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC864180.1724919165746552No Hit
CAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATT843890.16844199527666204No Hit
CAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTA838620.16739009358911033No Hit
TGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATC828150.16530026234268405No Hit
ATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTT817970.16326831562934888No Hit
CTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTA817190.1631126261955177No Hit
CTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCT809670.1616116203719145No Hit
AATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGG807540.16118646845645243No Hit
CTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCT786110.15690900106285982No Hit
ACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTT777830.15525629784219036No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG776580.15500679554438399No Hit
GTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTAT766070.15290898022442792No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA749280.14955766536029258No Hit
TATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACT748180.14933810333822298No Hit
GGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCA741490.14800276704036322No Hit
TACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGA725620.1448350858674134No Hit
TTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTG722750.14426222859164994No Hit
AGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTT720250.14376322399603716No Hit
AAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGG717760.14326621541880688No Hit
AACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCT716250.14296481664305677No Hit
GGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATA703770.14047378570175786No Hit
AACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAA703620.1404438454260211No Hit
ACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGAT698930.13950771280465155No Hit
TTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAG694480.1386194846244608No Hit
AGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCT691760.13807656762443413No Hit
TCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGC687700.137266184161159No Hit
TACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACA686050.13693684112805457No Hit
GGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAAT685980.13692286899937742No Hit
ATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACC684650.13665739855451142No Hit
ATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTA683100.13634801570523153No Hit
TATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTAC682790.13628613913537554No Hit
TTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGG668960.13352564571244574No Hit
GTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCA667700.1332741473962569No Hit
CAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGT666640.13306256944771708No Hit
GACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATT664840.1327032861388759No Hit
AGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAA661930.13212244478958263No Hit
CCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTT658480.13143381844763702No Hit
CTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAG656360.13101066255055738No Hit
AAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGA651980.13013640649904384No Hit
CTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGG649700.129681314307845No Hit
TGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAA649480.12963740190343107No Hit
AGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGAC645170.12877711798059466No Hit
TTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTC625220.12479506130760482No Hit
TAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCG623000.12435194522670068No Hit
GAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACA622580.12426811245463773No Hit
TAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTC618590.12347170112003976No Hit
ACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAG610790.12191480678172796No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT608200.12139783802067312No Hit
CTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAAC603850.12052957002430692No Hit
AAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTT597630.11928804659042236No Hit
AGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGG594870.11873714551686586No Hit
AGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCC594300.11862337246906615No Hit
AAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAAC593260.11841578655729125No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG592830.11832995776684585No Hit
AATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACT592450.11825410906831271No Hit
TGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTA588730.11751159023004092No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA587000.11716627904987688No Hit
CCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAA578680.11550559175567761No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT571400.11405249037325324No Hit
CAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCC568260.1134257406011636No Hit
GATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGCACAA567850.11334390384748312No Hit
CTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTA566560.11308641747614692No Hit
TCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGG562640.11230397827022612No Hit
GGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACC562270.11223012559007543No Hit
AGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAG561760.11212832865257043No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA561080.11199259940256376No Hit
ACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAG560680.11191275866726572No Hit
CCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTC559510.11167922451651893No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTG556510.11108041900178363No Hit
CTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGC555240.11082692466721233No Hit
CTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGT553900.1105594582039639No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTG553320.11044368913778174No Hit
TCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAA553220.11042372895395723No Hit
TCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGT550380.10985685973334114No Hit
TTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAG550350.10985087167819378No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA549630.1097071583546573No Hit
CAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGT546910.1091642413546306No Hit
TTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTG546880.10915825329948325No Hit
ATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCT542160.10821613262296637No Hit
AAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCG541640.10811233966707891No Hit
CTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTA540900.10796463430677752No Hit
TCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGA539550.10769517182514664No Hit
TCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCT536110.10700854150158348No Hit
GTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGAT531560.10610035313756824No Hit
AGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGC529920.10577300612284626No Hit
AACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTT526320.10505443950516388No Hit
GTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCT525390.10486880979559594No Hit
TTCTATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTC524680.10472709249044192No Hit
TTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAA524660.10472310045367701No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGAC521910.10417419539850298No Hit
ACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTT514110.10261730106019115No Hit
GTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTG513700.10253546430651066No Hit
GCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACC510730.10194264684692268No Hit
ACTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTT506810.10116020764100188No Hit
TTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTAC503370.10047357731743872No Hit
CTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGG501410.10008235771447832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)