FastQCFastQC Report
Thu 7 Jul 2022
EGAF00006219776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006219776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50099739
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC843860.16843600722151467No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT843030.1682703376957712No Hit
AAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTC793730.15842996707028753No Hit
AAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAG780120.15571338605177162No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA742360.14817642063963646No Hit
AAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAG734250.14655764973146865No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG727850.1452801979667No Hit
AGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGAC724730.14465744023137525No Hit
TAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGA716780.14307060561732668No Hit
TGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTC686790.13708454648835594No Hit
TGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATC682430.1362142824736073No Hit
TAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAA657750.1312881091057181No Hit
CAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCA637820.12731004446949315No Hit
CAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTA624900.12473118871936639No Hit
CAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATT623810.12451362271567923No Hit
TTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCT616140.12298267661633926No Hit
CTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTA598170.11939583158307471No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG595440.11885091856466558No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT586310.11702855378148776No Hit
ATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGC584250.11661737399470284No Hit
GTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTAT567580.11329001135115693No Hit
ATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTT566250.11302454090629094No Hit
TACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACA555140.11080696448338782No Hit
ACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTT552890.11035786034733633No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTG552870.11035386831057144No Hit
GGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCA551710.11012233017820712No Hit
CTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCT551680.11011634212305975No Hit
CTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCT546260.1090345001597713No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTG544890.10876104564137551No Hit
GATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGCACAA540880.10796064227001263No Hit
AATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGG535540.10689476845378376No Hit
TACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGA534610.10670913874421581No Hit
AGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTT533960.1065793975493565No Hit
AAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAGGCTCGG533230.10643368820743757No Hit
AGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCT526440.1050783917257533No Hit
AGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAA519720.1037370673727462No Hit
AACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAA514900.10277498651240478No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA510470.10189075036897896No Hit
TGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAA509750.10174703704544248No Hit
CTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAG507970.1013917457733662No Hit
AAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGA507390.10127597670718405No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA505680.10093465756378492No Hit
AAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTT505450.10088874914098854No Hit
TTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTG504070.10061329860421028No Hit
GGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAAT502390.10027796751595852No Hit
ACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGAT502130.1002260710380148No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)