FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006219795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006219795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67438383
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT12732561.8880286616599333TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTA914120.13554892026399862TruSeq Adapter, Index 7 (97% over 35bp)
CTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCT849120.12591049224890222No Hit
TATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACT781660.11590728680431144No Hit
AACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCT768010.11388321692114119No Hit
ATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACC729240.108134265318906No Hit
TATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTAC726680.10775465953861912No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA722340.10711110911422654No Hit
AAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACCAG718590.10655504595950943No Hit
GGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATA692000.10261218748379539No Hit
AAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCT679500.1007586436347384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)