FastQCFastQC Report
Sat 11 May 2024
EGAF00006223653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006223653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14739181
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCATTCCATCTCGTAT184193312.49684768780572TruSeq Adapter, Index 1 (97% over 37bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACATCATTCCATCTCGTAT9048786.139269203628071TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCATTCCACCTCGTAT514340.34896104471476397TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCATTCCAACTCGTAT358790.2434260085414515TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCATTCCAGCTCGTAT289230.19623207015369445TruSeq Adapter, Index 1 (97% over 37bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACATCATTCCACCTCGTAT242470.1645071052455357TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCATTCCATATCGTAT183880.12475591418546253TruSeq Adapter, Index 1 (97% over 37bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACATCATTCCAACTCGTAT168720.11447040374902785TruSeq Adapter, Index 1 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)