FastQCFastQC Report
Mon 8 Aug 2022
EGAF00006223659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006223659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28196263
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGAATCATCTCGTAT23394348.296964743164724TruSeq Adapter, Index 9 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGAATCATCTCGTATG812520.28816584665847383TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGAATCACCTCGTAT325230.11534507250127438TruSeq Adapter, Index 9 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)