FastQCFastQC Report
Mon 8 Aug 2022
EGAF00006223661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006223661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26131626
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTAT620221723.734523829477737TruSeq Adapter, Index 10 (97% over 38bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTAT1238100.4737937088185787TruSeq Adapter, Index 9 (97% over 35bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTAT1218010.46610570654883854TruSeq Adapter, Index 9 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAACCTCGTAT608880.2330050185166434TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAAACTCGTAT451110.1726299006422333TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAAGCTCGTAT382710.1464547211872694TruSeq Adapter, Index 10 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)