FastQCFastQC Report
Mon 8 Aug 2022
EGAF00006223662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006223662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26131626
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGAAGCCAGTGTAGATCT19329457.396956469528532Illumina Single End PCR Primer 1 (97% over 35bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCTCTTGTGTAGATCT17151856.563636721266406Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTACCACCAGTGTAGATCT5130311.9632570893215753Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGGATTGTGTAGATCT4955541.896376444389645Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATAGAGGGTGTAGATCT4586541.7551682394352346Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAATCTGTGTGTAGATCT4342501.66177948513422Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)