FastQCFastQC Report
Mon 8 Aug 2022
EGAF00006223664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006223664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30469369
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCAACATTGTGTAGATCT12896044.232460475305544Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTAGCCTGTGTAGATCT7886472.588327313243671Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAGACGGAGTGTAGATCT3391631.1131277447852628Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)