FastQCFastQC Report
Mon 8 Aug 2022
EGAF00006223669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006223669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28535675
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTAT388798013.624979959296565TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAACCTCGTAT557140.19524332261283464TruSeq Adapter, Index 10 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTATG468780.1642785741006652TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAAACTCGTAT449670.15758169379206904TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAAGCTCGTAT352960.12369078355427023TruSeq Adapter, Index 10 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)