FastQCFastQC Report
Mon 8 Aug 2022
EGAF00006223671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006223671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28412693
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTAT6018022.1180744817113957TruSeq Adapter, Index 7 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTATG4290591.5100962094652555TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)