FastQCFastQC Report
Sun 5 May 2024
EGAF00006223675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006223675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32016211
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTAT27405508.559882367092095TruSeq Adapter, Index 1 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTATG2081660.650189368129789TruSeq Adapter, Index 1 (97% over 37bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTAT642860.2007920300125458TruSeq Adapter, Index 5 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTACCTCGTAT351290.1097225402468768TruSeq Adapter, Index 1 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)