FastQCFastQC Report
Mon 8 Aug 2022
EGAF00006223676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006223676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32016211
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGTGAGTTGTGTAGATCT12425913.881130718435108Illumina Single End PCR Primer 1 (97% over 34bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACCTTAGGTGTAGATCT2501380.781285455671191Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGATTAGGGTGTAGATCT2255120.7043681714866259Illumina Single End PCR Primer 1 (97% over 36bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAGGTGCTGTGTAGATCT2035730.6358435106515259Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)