FastQCFastQC Report
Mon 8 Aug 2022
EGAF00006223677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006223677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21073853
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGGCTCATCTCGTAT4192511.989436862827125TruSeq Adapter, Index 12 (97% over 37bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGGCTCATCTCGTAT2139021.0150113507956993TruSeq Adapter, Index 27 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)