FastQCFastQC Report
Mon 8 Aug 2022
EGAF00006223681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006223681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18722183
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTAT17516759.356147197151103TruSeq Adapter, Index 10 (97% over 38bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTAT9727415.195660142836976TruSeq Adapter, Index 9 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAACCTCGTAT354630.18941701403089586TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAAACTCGTAT335540.17922055350062543TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAAGCTCGTAT265240.14167151341272544TruSeq Adapter, Index 10 (97% over 38bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAAACTCGTAT215080.11487976589054812TruSeq Adapter, Index 9 (97% over 35bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAACCTCGTAT211210.11281269924559546TruSeq Adapter, Index 9 (97% over 35bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTAT196610.10501446332406857TruSeq Adapter, Index 9 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)