FastQCFastQC Report
Mon 8 Aug 2022
EGAF00006223683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006223683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16393729
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTAT366265222.341786911324448TruSeq Adapter, Index 1 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTATG1259690.7683974768644767TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTACCTCGTAT626930.3824206194941981TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTAACTCGTAT422240.2575619006511575TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTAGCTCGTAT348350.21248978801589316TruSeq Adapter, Index 1 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)