FastQCFastQC Report
Mon 8 Aug 2022
EGAF00006223684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006223684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16393729
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGTGAGTTGTGTAGATCT15169469.253208955692752Illumina Single End PCR Primer 1 (97% over 34bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACCTTAGGTGTAGATCT3950072.4095006084338713Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAGGTGCTGTGTAGATCT2366871.4437654788608498Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGTCGTGTGTGTAGATCT2306471.4069221224774424Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)