FastQCFastQC Report
Mon 8 Aug 2022
EGAF00006223705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006223705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16101131
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTAT11091096.888391877564377TruSeq Adapter, Index 1 (97% over 38bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTAT6006793.73066339252814TruSeq Adapter, Index 5 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTACCTCGTAT274380.1704103891832195TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTAACTCGTAT220790.1371270130029996TruSeq Adapter, Index 1 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTAGCTCGTAT178840.11107294263986797TruSeq Adapter, Index 1 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)