FastQCFastQC Report
Mon 8 Aug 2022
EGAF00006223706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006223706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16101131
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGTGAGTTGTGTAGATCT3953372.455336833170291Illumina Single End PCR Primer 1 (97% over 34bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGATTAGGGTGTAGATCT1288740.8004034002331887Illumina Single End PCR Primer 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)