FastQCFastQC Report
Mon 8 Aug 2022
EGAF00006223719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006223719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3373959
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCATCTCGTATG34248710.150893949807926TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCATCTCGTAT1160713.4402018518897237TruSeq Adapter, Index 2 (97% over 38bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCATCTCGTAT661391.9602787111520918TruSeq Adapter, Index 2 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCAACTCGTATG426871.2651902409009712TruSeq Adapter, Index 2 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCACCTCGTATG177000.5246062563297301TruSeq Adapter, Index 2 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCAGCTCGTATG82320.2439863673506406TruSeq Adapter, Index 2 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCATCTCGTATT79730.23630992552073096TruSeq Adapter, Index 2 (97% over 37bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCATCTCGTATG73410.21757822190488976TruSeq Adapter, Index 2 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCATTCACCGCTGATCATCTCGTATG45900.1360419613872012TruSeq Adapter, Index 2 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCCTCTCGTATG45210.13399688615066158TruSeq Adapter, Index 2 (97% over 37bp)
ATCGGAAGAGCACACGTCTTAACTCCAGTCACCGCTGATCATCTCGTATG35080.10397281057653635TruSeq Adapter, Index 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)