FastQCFastQC Report
Mon 8 Aug 2022
EGAF00006223723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006223723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4242442
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGTAT43072610.15278464620141TruSeq Adapter, Index 8 (97% over 37bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGTAT2484225.855636918548327TruSeq Adapter, Index 5 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGTATG1405993.3141054138159105TruSeq Adapter, Index 8 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAAACTCGTATG249160.5873032560020857TruSeq Adapter, Index 8 (97% over 36bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGTAT71660.16891215012485733TruSeq Adapter, Index 5 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAACCTCGTAT68770.1621000357812788TruSeq Adapter, Index 8 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAACCTCGTATG65080.15340221504501417TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAAACTCGTAT57850.13636014352111356TruSeq Adapter, Index 8 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAAGCTCGTAT48120.11342523952006886TruSeq Adapter, Index 8 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)