FastQCFastQC Report
Mon 8 Aug 2022
EGAF00006223724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006223724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4242442
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGATGTTTGTGTAGATCT662701.5620720330413473Illumina Single End PCR Primer 1 (97% over 36bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGACCACTGTGTAGATCT556561.3118859373917193Illumina Single End PCR Primer 1 (97% over 35bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATCACGTTGTGTAGATCT357050.8416143343857146Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTAGGCATGTGTAGATCT350760.8267879678732202Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)