FastQCFastQC Report
Thu 21 Jul 2022
EGAF00006239895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006239895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54985143
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG7314961.3303520916550131Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3743260.6807766236053983Illumina Single End PCR Primer 1 (100% over 50bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA1716100.3121024892124042No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACA1408010.2560709899399552No Hit
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAAC874020.15895566553314228No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAAC708930.12893119146748422No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)