FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006239940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006239940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40075367
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC2021490.5044220805264241TruSeq Adapter, Index 4 (100% over 50bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA1149990.2869568231277832No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG961940.24003273631904606TruSeq Adapter, Index 4 (100% over 49bp)
TGGGTTTATGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA731440.18251610771275031No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA709090.17693911574159757No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC700490.17479315909945378No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT613480.15308156753748506No Hit
TGGGTGTGGTGGTTTATGTTTGTAATTTTAGTATTTTGGGAGGTTGAGGT613030.15296927910853567No Hit
TGGGTTTATGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA461300.11510811616522439No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA447820.11174445389358506No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA423060.10556609500294782No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)