FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006239941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006239941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40075367
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1687470.4210741226649278Illumina Single End PCR Primer 1 (100% over 50bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA1149540.2868445346988338No Hit
CAAATTCACACCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACA1130040.2819787027776939No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACA964570.24068899980379468No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC901440.22493618087140663Illumina Single End PCR Primer 1 (100% over 50bp)
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAAC691740.17260977298099353No Hit
CAAACACAATAACTCACACCTATAATCCCAACACTTTAAAAAACCAAAAC442440.11040198334303464No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAAC425450.10616247132559012No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)