FastQCFastQC Report
Sun 24 Jul 2022
EGAF00006239943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006239943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41038045
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACACCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACA1668970.40668847651002865No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1034460.2520734113917951Illumina Single End PCR Primer 1 (100% over 50bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA983440.23964104527883823No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC940220.22910935450263284Illumina Single End PCR Primer 1 (100% over 50bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACA812830.19806742743227654No Hit
CAAACACAATAACTCACACCTATAATCCCAACACTTTAAAAAACCAAAAC693690.16903583004502287No Hit
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAAC499060.12160910686656735No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)