FastQCFastQC Report
Sat 16 Jul 2022
EGAF00006239984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006239984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57832918
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA3922160.6781881557489455No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3075290.5317542510996938TruSeq Adapter, Index 4 (100% over 50bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT2772470.47939306814848937No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2652210.4585986825012011No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1800910.3113987781145679TruSeq Adapter, Index 4 (100% over 49bp)
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1688480.2919582926803036No Hit
CGGGCGTGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1297150.22429267705288536No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1186620.20518072423736253No Hit
CGGGTTTATGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA695710.12029654114979985No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)