FastQCFastQC Report
Thu 21 Jul 2022
EGAF00006239985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006239985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57832918
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA4892970.8460527618544166No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACA3753700.6490594162999004No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2148820.3715565588442209Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1491980.25798110342625286Illumina Single End PCR Primer 1 (100% over 50bp)
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAAC1310370.22657857243170748No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAAC969950.16771590186751428No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCTAAATAACTAAAACTACA790330.13665746556312447No Hit
CAAATTCACACCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA682840.11807116493758797No Hit
CAAACGCGATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAAC661490.11437949577436159No Hit
CAGATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA640540.11075699137297551No Hit

[FAIL]Adapter Content

Adapter graph