FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006239987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006239987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67235528
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA4601950.6844521247754609No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4212140.6264753360752964Illumina Single End PCR Primer 1 (100% over 50bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACA3550620.5280868769261394No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1983940.29507316429492453Illumina Single End PCR Primer 1 (100% over 50bp)
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAAC1425950.2120828143121No Hit
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAAC1240200.18445605127098877No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAAC1056370.15711485154098886No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCTAAATAACTAAAACTACA801340.11918401235727635No Hit
CAAATTCACACCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA750760.11166120388018667No Hit
CAAACGCGATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAAC717440.10670549058527509No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)