FastQCFastQC Report
Sat 16 Jul 2022
EGAF00006239988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006239988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57675435
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC8077291.4004731823869208TruSeq Adapter, Index 8 (100% over 50bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA3769080.6534983221192869No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2651630.4597503252467884No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT2160280.3745580765884124No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1352110.23443429598753784No Hit
TGGAGGAGGAGATTTTATTTTATATTAATATTTATTTTGATTTTTTGGTT1345960.23336798413397314No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG1326580.23000780141493515TruSeq Adapter, Index 8 (100% over 49bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1153870.2000626436540964No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA1056320.18314903043210684No Hit
CGGGCGTGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1004910.1742353568724709No Hit
TGGATAATGAATTATAATTAATATTATTAATTAATATTTATTATTAATAA838300.14534784176313537No Hit
CGGGTTTATGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA660460.11451322387078658No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG657670.1140294823957548No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)