FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006239989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006239989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57675435
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5527960.9584600445579649Illumina Single End PCR Primer 1 (100% over 50bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA4354810.7550545565889534No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACA3331160.5775699827838316No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAAC1377240.23879143694364854No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1146260.19874319110033586Illumina Single End PCR Primer 1 (100% over 50bp)
CAAAATAATAAATTACAATATAAAAAATTATTCCAAAACCTAATAAAATA1135090.19680649135979641No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAAC1012230.17550452805427474No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCTAAATAACTAAAACTACA714330.12385342217184837No Hit
CAAACGCGATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAAC661520.11469701095449042No Hit
CAAATATCAAAAAAATACCTTAAATAACCTCTAAAACTCAAAAATAAAAA640910.11112356586473948No Hit
CAAATTCACACCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA640010.11096752022763244No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)