FastQCFastQC Report
Thu 21 Jul 2022
EGAF00006240041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006240041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57945689
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA5633300.9721689563480728No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC5023760.866977351844069Illumina Single End PCR Primer 1 (100% over 50bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACA4291570.7406193754983222No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3072480.5302344407363937Illumina Single End PCR Primer 1 (100% over 50bp)
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAAC1445450.2494491005189359No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAAC1085460.1873236851148668No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCTAAATAACTAAAACTACA889110.1534385068749463No Hit
CAAATTCACACCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA800220.13809828027068588No Hit
CAAACGCGATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAAC712740.12300138496929426No Hit
CAAATTCACACCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACA614950.10612523737529465No Hit
CAAATTCATACCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA600300.10359700788094865No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)