FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006240123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006240123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59424292
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA4182450.7038283266378672No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACA3214290.5409050561342826No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1877270.31590952736971606Illumina Single End PCR Primer 1 (100% over 50bp)
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAAC1286980.21657473007839959No Hit
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAAC1214940.20445174172205538No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAAC929950.15649324017188124No Hit
CAAATTCACACCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACA889610.1497047705675652No Hit
CAAATTCACACCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA749160.12606965515045598No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC692170.11647930109121031Illumina Single End PCR Primer 1 (100% over 50bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCTAAATAACTAAAACTACA684440.11517848626618893No Hit
CACATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA669740.1127047504411159No Hit
CAAACGCGATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAAC635820.10699664709509706No Hit
CAAAATAATCTCGATCTCCTAACCTCGTAATCCGCCCGCCTCGACCTCCC600150.1009940513889505No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)