FastQCFastQC Report
Sat 16 Jul 2022
EGAF00006240133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006240133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60671103
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC6405531.055779388088593Illumina Single End PCR Primer 1 (100% over 50bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA4381680.7222021330319313No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACA3133330.5164452012682216No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1588260.2617819557359951Illumina Single End PCR Primer 1 (100% over 50bp)
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAAC1432290.2360744949700354No Hit
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAAC1192230.19650705872283217No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAAC1051330.1732834822534873No Hit
CAAACGCGATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAAC733000.12081534103640738No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCTAAATAACTAAAACTACA718990.11850616923842641No Hit
CAAATTCACACCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA638660.10526592865799721No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)