FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006240205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006240205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47560115
Sequences flagged as poor quality0
Sequence length151
%GC38

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5010041.053412086997687Illumina Single End PCR Primer 1 (100% over 50bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA1446750.30419396588927505No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACA1172330.24649435771969852No Hit
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAAC1056290.22209576238409012No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC983610.2068140499660272Illumina Single End PCR Primer 1 (100% over 50bp)
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAAC542030.11396734427576553No Hit
CAACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCGTATTAACC483630.10168814772630386No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)