FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006240207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006240207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48300554
Sequences flagged as poor quality0
Sequence length151
%GC39

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG14396412.9805890011116642Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC7538841.5608185363670983Illumina Single End PCR Primer 1 (100% over 50bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA1587380.32864633395302256No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACA1276630.2643095977739717No Hit
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAAC668240.13835037999771183No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAAC583650.12083712331746753No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)