FastQCFastQC Report
Thu 21 Jul 2022
EGAF00006240265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006240265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49826006
Sequences flagged as poor quality0
Sequence length151
%GC38

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1929230.3871933865218898Illumina Single End PCR Primer 1 (100% over 50bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA1698040.34079392195312624No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACA1401700.2813189562093337No Hit
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAAC793700.1592943251361548No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAAC685370.1375526667740537No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAAC500700.1004896920696393No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)