FastQCFastQC Report
Mon 8 Aug 2022
EGAF00006414808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006414808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53609839
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA563824810.517188831699345No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG36503796.809158669549446No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC8955991.6705869980322081No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT8689141.6208106873814712No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAG1410810.2631625138810807No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA951020.17739654095958018No Hit
CACACACACACACACACACACACACACACACACACACACACACACACAAA948450.17691715134604302No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG933110.17405573629870444No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC711680.13275175103584996No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGTG660700.12324230259299976No Hit
CACACACACACACACACACACACACACACACACACACACACACACAAACA586380.1093791757143684No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)