FastQCFastQC Report
Mon 8 Aug 2022
EGAF00006414910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006414910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5669203
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA72087312.71559688372422No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG59318510.463287343917655No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC2416024.2616572382396605No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1387562.4475398040959195No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA421490.7434731125345133No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG333640.58851305906668No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC205030.3616557741890703No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAG150360.2652224660150642No Hit
CACACACACACACACACACACACACACACACACACACACACACACACAAA87300.15398989946205843No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGTG76000.13405764443432347No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT67450.1189761594354621No Hit
CACACATACACACACACACACACACACACACACACACACACACACACACA63490.11199105059388419No Hit
ATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT60270.1063112398691668No Hit
CACACACACACACACACACACACACACACACACACACACACACACAAACA60250.10627596154168406No Hit
CGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG60100.10601137408556369No Hit
CACACACGCACACACACACACACACACACACACACACACACACACACACA58410.10303035541327414No Hit
CTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT57900.10213075806246485No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)