FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006415008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006415008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10436
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGCATGTATCTCGTAT8698.326945189727866TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGCATGTATCGCGTAT3503.3537753928708316TruSeq Adapter, Index 7 (97% over 36bp)
TAGAAAGAACTACTTTTTAAAAAATGGGTGGAATAGGGGGCAATCTGAAA620.5940973553085473No Hit
GGCATGGTGACAGCGGTGAGAGGCTGCAGGTGCACAGATCTCAACGAGGG610.584515139900345No Hit
GCTTATAATAATAGAAACACATTCTGTCACAGTTCTGGACAGCAGAAGTC540.5174396320429283No Hit
TTTTTTTTTTCTAGCATTGTTACCTTGTGGGCCATACAAAACAAGCAGCT430.4120352625527022No Hit
AGGAAATATTTCAAAATCTTGTTTCTCTGGTTTTTACCAATAGATGTAAG400.3832886163280951No Hit
TAAAGGGCTTTTTACTGCACCTATTGGATTGTACCACTTCCATCAATAGC380.3641241855116903No Hit
TTTCCCATCATTTCCACCCACCTGCTCCAGCATCCTCTGGCAGAAGAAGG370.3545419701034879No Hit
ATGCGGGGAGCTCGGATGTCCGGTTTCCTGTGAGGCTTTTACCTGACACC330.3162131084706784No Hit
TTCACAGGTGCGATCATAGAGCACCACCTCCTGGAACTCCTGGGCTCAAA330.3162131084706784No Hit
GTGGTCTGGCTGTGTGGCCTTAGAGTGGTGGCTTCACCTCCCTTCACACC320.30663089306247604No Hit
ATTTATATGTGGAAAATCAATCCAGGGTTTTTAGCTCAGTTTTGGTTCAT320.30663089306247604No Hit
ATTTTTGTAGACTCAATTATCACGTACTGAGGCTATACTGCCCTCTTGTG320.30663089306247604No Hit
CGGCTCTCGCTCCGAGGGGGCGTGTGTTTGTGTCTGTGGGGAGAGTACCG310.2970486776542737No Hit
TTCACATTTATTTATCAGTGTCCCAGTAGAGGGCATTGTTAATGCATGGG300.2874664622460713No Hit
AATTCCCCAACTCTAGCAGTGTTATGACAGGATGCTGCCACTAGGTGGCT280.2683020314296665No Hit
GGTCCTTAGTAACAGACCCTCAGTTGAGCCACCTACTGGGTGCTACTAGC280.2683020314296665No Hit
TAACATTACGTGATTGTGAAATAGCTGCCTGCTCTAGAAGGTCCTTGTTC270.25871981602146416No Hit
AACTGGTTTTGGTGGTGGTGGTGGTTGGTTAAAAGTCAACTATTGCACTT260.2491376006132618No Hit
TCTTATGAGAATCTGATGCCTGATGATCTGTCACCGTCTCCCATCACCAC260.2491376006132618No Hit
AAGAGACAGAGAAGGGGGTGGCCTCTCGTCTCCCTGGGGACCTGCCATTC250.2395553852050594No Hit
CCTCAGATCATCAGGCATTAGTTAGATTCGCATAAGGAGTACACAACCTA240.22997316979685703No Hit
AATATGCCTTTAGTAGCATCACTAAGAAATGACTCAGAAATTATACCTCA240.22997316979685703No Hit
TTAAATCTGTCTTGCTTATTGGGAGTCAAAGCTGCAACATAAAAGAACAA240.22997316979685703No Hit
CTTTATACAGTAGCATTATCTACACTTATTTCTAGCTCAACTATACGGAC230.22039095438865464No Hit
AGTAAATCATGCCATTGACCACATGGGGCTGCTGTAAGAATTAAATGAGA230.22039095438865464No Hit
AAATAAAAGTTCTTACATAAGATGGCTGGTTTTGTATTTGAAATTACTGA230.22039095438865464No Hit
ACAGCTCTAAGTGAATAATCACAATTTAAAGAATGAAGTCTAAATTACTG230.22039095438865464No Hit
CCTTATTTGATGTTCAGGGGCATCCACACTTTCTCTTTCCTGCTGGAAGG210.20122652357224988No Hit
CAAGCCACCGCACCCCCCCTTTAAATACCATGTCGGGGCCCCCCTGGAGC210.20122652357224988No Hit
AAACATTTCTTCTAGGATGTTGCCTTTCTCCCCAAGCCCCAGTTCTCTCT200.19164430816404754No Hit
CTTGCATCTGGGTCACGCTGGCACAAGAGGTGGGCTCCCACAGCCTTGAG200.19164430816404754No Hit
TACAGAACCTTTTGTTAGTCTCAATTTCTGGTTATTTCATTTCAATAAAA190.18206209275584515No Hit
ACCAGCCTTATTGCTGATATGGAGAAAATTGTAGTGGTCTGGATAGAAGA180.17247987734764278No Hit
CATGGTGCAAGCGGTCAGTGGATCTACCATTCTGGGGTCTGGAAGACAGT180.17247987734764278No Hit
GCTTCCACAAGATGGCAGCAAAGCTTTGGAAAGCTACCCTTACTTTTTCC180.17247987734764278No Hit
CTTTCCTGAATGTAATTTCATACCTGGGATCCACTTGATTTCTGTTCAGA180.17247987734764278No Hit
GTAGAAATGAGGCCTTCTGGAGTTGTGCAGTAGGGGGTGCCCTGCACAGG180.17247987734764278No Hit
AATTCATACTACGGTTCCCATGGAGAGCTTCCTGCCCTGGAGCCTGCGAC180.17247987734764278No Hit
GTTAGATAACAGGCGGTATTATTATCAGGATTCAGCAGTGCAATTGATTC180.17247987734764278No Hit
TCAACTTCCGAGGCTCAGCACCCTCATGTGGGAAATGGGACTCCCACACT170.16289766193944039No Hit
GGTTTTTTATTTGGTGGTGCTATTTGGTTGCCATTCCAGTAGGTGGCACT170.16289766193944039No Hit
TATAAAATTTTCTTTTTAATTCTCAGCAAGGCAAGATACTTCTATAGAAG170.16289766193944039No Hit
ATGTTCCCTGGGAGACACTGTGCTTTTCTGCCACCTTGTGGTAGCACAGG170.16289766193944039No Hit
GTCATGAGAATGGCCAAGGAAGGGCTGTTGTTGGTTTATCAGTAGCACCC170.16289766193944039No Hit
AATTCATTTATTTTGCGTGTGCGCTGACAGTGAAGGGCTGAAAGCAATGC160.15331544653123802No Hit
TCTGGATTTTCTGCCAATGACTTGCACCTCCACCTGCTGGTGTACTGAGG160.15331544653123802No Hit
TGGCAGAACCACCTGAGGAGTATTTCAAAGTACACGTGCCTGGGCCCCCA160.15331544653123802No Hit
TTTTCCAGGTGTTTGCTTAGGAAGCACTGCCATCTTGTGGCTTACTATAG160.15331544653123802No Hit
ATATTTTAGAGTAAATGGAGGGAAAGTTTTAACATGCAGCACCACCTAGT160.15331544653123802No Hit
AGCTGTTGTGTAAATTGTAGGGTGTGTAGTAGCATCCCTGGACTCTACTC160.15331544653123802No Hit
ATTTCTGTTTAACTGTTGCTTCGGTTCTTCATTTCCTTATGAAGGCTCCG150.14373323112303565No Hit
ATAATCCTTGCCAAACGTCCAATATCATGGAAACTGCTAGTGCTCCATTT150.14373323112303565No Hit
ATATAAATTTATCTTGTACGTTGTCTAGAAATTTTCACCAGGAGAGGGCA150.14373323112303565No Hit
AAGAAGCGCTGCTCCCAGGTGTGCTTGACCTGCAAGATTGGCCCCTGCTG150.14373323112303565No Hit
TGTATTGTGCTTGTAGGTTGTTTAGCAACATCCTTGGCCTTTACCCACTA150.14373323112303565No Hit
TATGTATGTACTTTTTAAGGTTTTTCAGAATTTAAACACGACATGAGCGC140.13415101571483326No Hit
AAGCATGAGGGATTAATCTTGAAGCCGGTCTTGGTATCTAAGGGCTGCCC140.13415101571483326No Hit
AATTACCATGAAAGCACAGCCATTTCACTTGAACATACATAAATAACCCA140.13415101571483326No Hit
AAAACATTTCTGTTTAACTGTTGCTTCGGTTCTTCATTTCCTTATGAAGG140.13415101571483326No Hit
ATTTCGTAGGGCTGAGGGTCAGAAGAGAGGGGAAAGCGGTCATAAGAAAC140.13415101571483326No Hit
AAATTTTTAACATGAAAGTTATACTATACTGCCTCCTAGTGTACAGTTAA140.13415101571483326No Hit
TTTATTTATTTATTTATTTATTTATTTATTTTTTCTGTAGCTGTCACTTA140.13415101571483326No Hit
CTAGATTTATTGCTTGGAAATTATAATGCATAGTGGGCCTTTCAAAAAAA140.13415101571483326No Hit
GCAGCGGGACCCTCCTGCTGGCCCACGTGGCTGCCACAGCCCATGCTGGC140.13415101571483326No Hit
TTAAGTGTATTTTTGAAACATACTTAGAGCATTTTGAAATGCTTTTTTGA140.13415101571483326No Hit
AGACTGGCCTCAAACTCCTGGGCTCGAGCAGTCCTCCCACCTCAGCCTCC140.13415101571483326No Hit
ATTTTCTTGCTCTCGGTGCCACGGTTCGCCTGCCTCGACATGGTGCCGGC130.1245688003066309No Hit
TTATAGACATACCAGCAGGGGGTGAGGGAGCAGTGCTGACCTGTGGGAAG130.1245688003066309No Hit
TGGACTCAGGGCTGAGCGGCAGGGGGCGCTCCTCCCAGTGCAAGCCTTCA130.1245688003066309No Hit
TTAGAAAACTCAAGCAGTTATTTTGGAGAATAAGGCACCTCCTCGTGGGG130.1245688003066309No Hit
AAAAAATCCAATTTATTTCTTCAAACTTTCCACTAGGTGGTGCTAAAGTG130.1245688003066309No Hit
ACATGCTGGCCCCACGACCTCGGGCATGGCCTTCCCTGTCCAGCCTCACG130.1245688003066309No Hit
ATCGAGCTTTTCTTTCAGCTTCTAGGCCTGCCCCTTCCGCTTTCTGAAAT130.1245688003066309No Hit
AAGTAGGAGGCTTCCTCAACACAGCCCGGGGTTCTTTCCTCCGCTCCTGC130.1245688003066309No Hit
ATGAATATGTATGTACTTTTTAAGGTTTTTCAGAATTTAAACACGACATG130.1245688003066309No Hit
ATAAATCTCTCCCAGGAAAGACCACCCGGTGGCAGTGTTAGGCACAACTT130.1245688003066309No Hit
CAGTAATCTAGAATCAGTGGGAGCCCTGAGCTTGTTTTCCTGCAACTAGA130.1245688003066309No Hit
ATTGAACAAAAAGAAAAATCATCTCTGACCAGCGGGGACAAGTCCCGGCT130.1245688003066309No Hit
AGTAAGAAGTATTTTTAAATCTTTTAAAAATAAGCAGAAATTAAAGCCCA120.11498658489842851No Hit
AGGCCACAGGGAGGCAGTAGTGAACGGCACAGTCTCTGCTCCCCAGGAGC120.11498658489842851No Hit
TTTAAGTAAAACATTTCAGGCTAAGAATTAACCATTGTAAGGAATATGTC120.11498658489842851No Hit
GTAAAAATAAGTCTATTTTTAAACAGAGCCGATTTCTTCAAGTTGTGTAC120.11498658489842851No Hit
CCTTAAATGAGAAGGGCAGCCACAGACATCTGCCCACTGGAACTCTCTGG120.11498658489842851No Hit
ATTGAACAGCGGTGTGTTAGCAGCTCAGCTGGCTCCTGGCCCCGTCATGT120.11498658489842851No Hit
ATTTGTGTGATAAATACTTCAATGTCCACTTCTTCCACGAGCCCCTGAGC120.11498658489842851No Hit
TTAGTTCAGAGACTTGGAGCAGTGCCTCCTGGAGGTGGCAGAGCCCTTAG120.11498658489842851No Hit
TTAACAAGTGGTCCCTGGCTATGATAAACTTTAATTAACTGCACTATCAT120.11498658489842851No Hit
GATTTTTTAAAAGGCAATTTAATTAATTTCACTTTAGCACCACCTAGTGG120.11498658489842851No Hit
AGTATGGAAACTCCACAAGGTGGCAGTTATGCATTGCAAAGGACACCAGC120.11498658489842851No Hit
ATGTATTAATTAGAAACTAAGGTGTTTTTTTTCCCCTCCCCACAGGAAAA120.11498658489842851No Hit
TAGTGATTATTTTCTGTAGCGTTTTGACCTAAGATCTCAGCAATTTGAAC120.11498658489842851No Hit
ATCCCTCTCAACCCTGTGGGACATATGTGGTTTCCTCTCTAGAAAACTGA110.10540436949022614No Hit
TATTATATTGTATGCATACACTACATTTTGTTTATCCATTTACCAACTAT110.10540436949022614No Hit
TGTTTTCTGAGCACAGCTCATGCCCAAAGTAGGAGGATGTGGTCACCCTA110.10540436949022614No Hit
ATATGGCATCTCTGCAGCTGAAGGTTAAAAATCTGGGAATGACAGTTGTC110.10540436949022614No Hit
ATTGGTTCCATCTACCGAATGGGATATGAGGAGGCAAACCTTACCCTCCC110.10540436949022614No Hit
AAATCAGACCCTTTTAATTGAAAATAAAACCAAGCAACCCCGAAGGCTCT110.10540436949022614No Hit
AATGTAGAAGACGCCCCCTTGTGTTTGATCTGTCACGGTGAAACACATTT110.10540436949022614No Hit
ATTTCCACAAACGTGGTCGGTAGGTAAGTTAAGCCCTTAACCAATCCACT110.10540436949022614No Hit
TATTGTTTGAGACTGGGATTTGATTTTCCTGGACGCTAAATAAAACCTAG110.10540436949022614No Hit
TTTGTTTTTCTGCCCAAGGGTACCGCATACTAACGTTTTTGATTCACAAA110.10540436949022614No Hit
GTTTAAAACAGAATCGAGGCCCTGTCCGAACCCGCGGAAGAATCACTGAT110.10540436949022614No Hit
TAGCAATAGACATTTTATTAGAAAAAGAATTTTACTGCCATCTGCTGGAA110.10540436949022614No Hit
ATGTTTTGAGAAATGCCTTTGCAGCTAACATGTCTTTACTACCTTATTTA110.10540436949022614No Hit
TTATACATTTATTGGAGTAATTATTTCCTGTCTGTCTCCCCTACGAGATA110.10540436949022614No Hit
GTATTATTACCACTTGGTGGCAGAAAATCCTAATATATAATTGTTTCCAT110.10540436949022614No Hit
GATGGCAGCCAATTCACACCATTTTGGAGTCAAGGCTCTTGATGTTTGAG110.10540436949022614No Hit
AAAACAAAAGGAAACGCGTTTCCCTCTCTTAACTCCTGTCGGTTCCTTTT110.10540436949022614No Hit
ACTATAGAAATCATCACTGAGAGGATAGTCTTTCCAGTTGCTTTTAATAC110.10540436949022614No Hit
TCTTAAGTACCGACACTGTTTTAGAAATATACTCCTTCAACCAACAGAGG110.10540436949022614No Hit
AATTCCATTAATTTATCTGGCTTTCCCATGTTACCAGAGAGACTACAGAT110.10540436949022614No Hit
ATCTCCAGGTAGTGTTGGAATTAATTAATTAGAGGACACCCAGCTGGTGT110.10540436949022614No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)