FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006415009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006415009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10436
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG740.7090839402069758No Hit
TAGAAAGAACCACTTAAACTGTTAAATAGGAAGGAAAAAACATGTAGGGA600.5749329244921426No Hit
GGCATGGTTTCTGTGTCTCAGGAAGGGCTCTGGGCTTTCGGGCAAGTCTC590.5653507090839403No Hit
GCTTATAATAATAGAAACGCCAAAAGCCACCCAGAGGCTGGAAGAGGCAA560.536604062859333No Hit
AGGAAATATTTCAAAATGCTGTCCTGGATTATTATACTTTTCCACAAGAG370.3545419701034879No Hit
TTCCCATCATTTCTACCACAAGAGGGTGCCCTGTACGTGAAGCATTTTTG360.34495975469528556No Hit
ATTTTTGCAGACTTCTAATTTAGAATAAAGTTTCTGGGCACTCTGCACCA350.33537753928708314No Hit
ATCCCAGCTACTCAGGAGACTGAGGCGAGAGAATTGTTTGAGCCCAGGAG340.32579532387888077No Hit
ATGCAGGGCGCCCTCCCAGCCAGTGCCGGGGAAAGGCGGCGGGTGTCAGG330.3162131084706784No Hit
ATTTATATGTGTAAAATTAAGCCAGTAATCTTGGTCTCTCAAGGCATGAA320.30663089306247604No Hit
TAAAGGGCTTCCCCCTTCTGGCCACAAATGGTCAACAATGATTTTTGGTA310.2970486776542737No Hit
TCACATTTATTTATCAGGTGAGGATCCAGGAGGCCAGCATCAGTAGGACC300.2874664622460713No Hit
TAACATTACGTGATTGTAAAAGTAAAGCACTGCCCCCTAGAGGAACAAGG300.2874664622460713No Hit
GTGGTCTGTCTGTCCATCCCTCCAATAGGCTTGACGGCCCACCATATATA290.27788424683786894No Hit
TTTTTTTTTTCAACCATCTACACCAGGGGTCAGCAAACTATGGGCTGATG290.27788424683786894No Hit
TGTCGTTTGTGTTGGGGAGGGAGCTGGGAGGTCTGCTCTCCCGCTCCATC290.27788424683786894No Hit
GGTCCTTAGTAAATGTCCTTGCAGTGTTTGCCAACCTGATGGTGGAGGCT280.2683020314296665No Hit
AATTCCCCAACTCAACGCAGAGCCCTTGCCAAGGAAATTGTCAAAGTTAG270.25871981602146416No Hit
AATATGCCTTTAGTAGCAGTGACACCTCCTGGCTTGGCAAGAGAAGACTG250.2395553852050594No Hit
AACTGGTTTTGCTGGAGTAGGAGCTGGTTTGGCATATCTAATAAGATAGC250.2395553852050594No Hit
TCTTATGAGAATCAGTTGGAGCCCTGAGCTTATTTTCCTGCAACCAGACG250.2395553852050594No Hit
AGTAAATCATGTCATCAACCCCAGCCCTTCGGGGTGCTGGCTGTGCTAAG250.2395553852050594No Hit
ACAGCTCTAAGTAAATGAGAGCCTTGAATGCCGAACTCAGTAATTTAGAC230.22039095438865464No Hit
CCTCAGATCAACTGCAGCATTAGATGCTCACAGGAGCACAAACCCTATTG230.22039095438865464No Hit
CTTTATACAGTGGAATTACTTGCACTATGTAAATTATTTCTCCATATAGT220.21080873898045227No Hit
AAATAAAAGTTCTTCCAATAGAGGGCACTCTGATTCAGTAATTTCAAATA220.21080873898045227No Hit
TAAATCTGTCTTCAATTGACCACTAGATGGCAACATTGTTCTTTTATGTT220.21080873898045227No Hit
AAGAGACACAGAAGGGAGGCTGCTGCCTGCCATGCGCCTGTGCTGAGAAT220.21080873898045227No Hit
CTTGCACCTGGAAAAGCTGCAGACAATGCTGGCCCATGAAAACAGCCAGG200.19164430816404754No Hit
AAACATTTCTTCTAGTATATTGCCATCTAGAGGACCAAGTGCAAGCTACA200.19164430816404754No Hit
TACAGAACCTTTTGCTTTCTGCTTTTCTTTGAATGCCCTCTAGTGGAAAT190.18206209275584515No Hit
GTCATGAGAATCGCCACAAGGGGGTGCTACTGATAAACCAACAACAGCCC190.18206209275584515No Hit
ATTTTCTTGCTCCGGCGTCTGCAGGGGTCGCCGAGCTAACCCGTGGCTAG190.18206209275584515No Hit
CTTTCCTGATGTCAGCTTTGGGTACTGGGCACTTGAATGTTTCTGAACAG180.17247987734764278No Hit
CATGGAGAACCTCTGCTAGGAAAGTGTGGAAGGGAAATGTGGGGTCAGAG180.17247987734764278No Hit
GTAGAAATGAGACATTGCCGGGTGCAGCAGGGAAAGAGTGGAGGGGCTCA180.17247987734764278No Hit
CCTTATTTGATGTCCAGGGCCAGGTAGGGCTAGGGCCCCTGGGGAAGGGA180.17247987734764278No Hit
GGGCGTCATGGGGTGCGGGGCGACGGCGAAAGGCGGCGACGGCGGTGCTA180.17247987734764278No Hit
ATGTTCCCTGGCAGAAAAAGACTCTGTGGGCTTTTTGGCTCCCTGTGCTA180.17247987734764278No Hit
AATTCATACTACGGCTTCTTGGAGGGGAGCGGGGAGCAGGGGCTCCGAGG180.17247987734764278No Hit
ACCAGCCTTTATAGAACTGAAAAGAGCCCGCTTGCTCTGAATTAGATTTT180.17247987734764278No Hit
ATATAAATTTATCTTGTAAAGTTGTGCCCTCTCCTGGTGAAAATTTCTAG160.15331544653123802No Hit
TCAACTTCCTGAGTCCCCTGAGTTACCCGGTGAGGCCAGTGTGGGAGTCC160.15331544653123802No Hit
GCTTCCACAAAATTCGTTTTGGGCAAAGTTGGAAAAAGTAAGGGTAGCTT160.15331544653123802No Hit
GTTAGATAACAGTGCTATATTTCGTGGACACATGAGCAGGAATCAATTGC160.15331544653123802No Hit
CTGAATTTTATTTCCACTTCATTTCCTCAGTACACCAGCAGGTGGAGGTG160.15331544653123802No Hit
ATTTCTATTTAACGTAAGTTTTACGTGATGCGGAGCCTTCATAAGGAAAT160.15331544653123802No Hit
ATATAAAATTTTCTTTTTAATTCTCACCATTGCTCCATCTGTAAGGGTGC150.14373323112303565No Hit
AATTCATTTATTTGGCACTAGGGGGCGATATAGCATTGCTTTCAGCCCTT150.14373323112303565No Hit
AAGAAGCACTATGCCCGGTGGCCAGCAGGGGCCAATCTTGCAGGTCAAGC150.14373323112303565No Hit
TATGTATGTCTCATTTTAACCAGCAGGTGGCGCTCATGTCGTGTTTAAAT150.14373323112303565No Hit
TGGCAGAATCCCCCGGGGCACAAAAGAAACGTGATGGGGGCCCAGGCACG150.14373323112303565No Hit
AGCTGTTGTTACAACTAGGGGGTGCTACTAGCATCTTGGGAGTAGAGTCC150.14373323112303565No Hit
TTTTCCTTCTGTCTGCAATAACTATAGTAAGCCACAAGATGGCAGTGCTT150.14373323112303565No Hit
ATAATCCTTGCGCCCCCGTCTGCAAAATGGAGCACTAGCAGTTTCCATGA150.14373323112303565No Hit
GTAAAAATAAGTCTATTTTCAAAAGGACTTTTCAAAACACAACTTGTACA150.14373323112303565No Hit
TTATTTATTTATTTATTTATTTATTTATCTTTTTCTGTGGTAAACAGCGT140.13415101571483326No Hit
ATCGAGCTTTACTTTCTGCCTCAATCTCTACCTCCTTTGCATTTCAGAAA140.13415101571483326No Hit
AAATTTTTAAAATGAAAGTTTCTATTTTATGTTAACTGTACACTAGGAGG140.13415101571483326No Hit
GCAGCGGTAGCGTGGCCCGCAGGGACGCCGACATGCTGGAGCGTCGTGCC140.13415101571483326No Hit
GTATTGTGCTTGTTGCAACTGAAGGGGATCACTACTGGCATCTAGTGGGT140.13415101571483326No Hit
TGGACTCAAGGCTGGTGTTTCTGAAGGCTTGCACTGGGAGGAGCGCCCCC140.13415101571483326No Hit
TAAGTGTAATTTTGATTTAAAAAACGCTTTCAAAAAAGCATTTCAAAATG140.13415101571483326No Hit
TGGAGGTCACCTGCAAAGGTCACATTGTCTTTTCAGCAGGGCAGTTCAGG140.13415101571483326No Hit
AATTACCATTTCAAGTTTCTTCCCTTTACTTTCATCAAACTGGGTTATTT140.13415101571483326No Hit
GAGCCGGCGCCCGCACATCCGTAGTTTCTTATGACCGCTTTCCCCTCTCT140.13415101571483326No Hit
ATAAATCTCTCCCAAGGAAAAGTTGTGCCTAACACTGCCACCGGGTGGTC130.1245688003066309No Hit
TTATACATTTATTGGAGCTTATTATCTCGTAGGGGAGACAGACAGGAAAT130.1245688003066309No Hit
TTAAGTAAAACATGTTTCACTATACTGAAGAAATTTTTGACATATTCCTT130.1245688003066309No Hit
ACATGCTGGCCTTGGCCAACACCTCTGCAGCCAGGGGCGCCTGATGGAGC130.1245688003066309No Hit
GGTTTTTTATTTGCTGGTTCTTATTCTTAAGTGCCACCTACTGGAATGGC130.1245688003066309No Hit
ATGTTTTGAGAAATGTCTTCTCCTGGGCATAAATAAGGTAGTAAAGACAT130.1245688003066309No Hit
ATTGAACAAAAAGAAAACCCATCTCCAAAAGACCTGTTCGTCCCAGCCTG130.1245688003066309No Hit
ATGAATATGTCTCATTTTAACCAGCAGGTGGCGCTCATGTCGTGTTTAAA130.1245688003066309No Hit
AAAAAATCCAATTTAATTAATTTCACTTTAGCACCACCTAGTGGAAAGTT130.1245688003066309No Hit
AAGTAGGAGGAAGCGAGACAGCAGGAGCGGAGGAAAGAACCCCGGGCTGT130.1245688003066309No Hit
ATTTTCCTTCCCTCTCTGATGGGGAAAAATGGCCACCTGAGGGAAGTATA130.1245688003066309No Hit
AAAACATTTCTGTTTTACGTGATGCGGAGCCTTCATAAGGAAATGAAGAA130.1245688003066309No Hit
ATTTGTGTGAGAAAAACTCGGTGTTGTCCAGGCCCTCCAGGGGCTCAGGG130.1245688003066309No Hit
TTAACAAGTGGAACCCAGGCTAGAAGTGAATTATGATAGTGCAGTTAATT120.11498658489842851No Hit
CCAATGAGGAGTAGCAGAGGGAGGCTGAAAGGACACGGGAGGCCTCAGGA120.11498658489842851No Hit
CCCATCTGGCCCACCGGGTCTGAATAAATATATATGGCCTGAAAGAATAA120.11498658489842851No Hit
AGGCCTCAGAAGAGCTGAGTGAAGGGCCTGAGCACTTAGCTCCTGGGGAG120.11498658489842851No Hit
ATTTCCACAAACGAAGAGGATAGGTAGTATCTCTCAAGTGGATTGGTTAA120.11498658489842851No Hit
ATTGAACAGCAGAGGGCAGCCACCCCACATGACGGGGCCAGGAGCCAGCT120.11498658489842851No Hit
TATTGTTTGAGACCGGGACACGTAAAGGTGAATTCTAGGTTTTATTTAGC120.11498658489842851No Hit
GTATTATTACCACTTGGATTTCCAGACATTAAATAATTGTACATGGAAAC120.11498658489842851No Hit
TAGTGATTATTTTCTGCAGACTGACTTCGGAGTAATTCTTGAGCCAGGAG120.11498658489842851No Hit
AGCCATGCCTGCAAGCCCAGACCAGACTCTTCCTGCCCCAAGTGGCCGAA120.11498658489842851No Hit
AAGCATGAGTGGGACCCAGAGGAGGGCAGCCCTTAGATACCAAGACCGGC120.11498658489842851No Hit
GATTTTTTAAAAATCCAATTTATTTCTTCAAACTTTCCACTAGGTGGTGC120.11498658489842851No Hit
GCCATACCCATCAAAAACAGAAACTTTCCAACCAGGCAGGTGGGCAGATT120.11498658489842851No Hit
CAGTAATCTAGAATCTAATCCCTGGTGATCTTTCACTGTCTCCCATCACC120.11498658489842851No Hit
CCCTAAATGTGGCCACCAGAGAGTTCCAGTGGGCAGATGTCTGTGGCTGC120.11498658489842851No Hit
ATATTTTAGAGTAAATGAGATGGTGAGTTTAACGCACCACTTTTCCACTA120.11498658489842851No Hit
AGTAAGAAGCCTTTTTAAAGAACAGAAAGAGTGGGCTTTAATTTCTGCTT120.11498658489842851No Hit
TAGCAATAGACATTTCATATAGTTATGAAGACAACTTTCCAGCAGATGGC110.10540436949022614No Hit
AGTTCGTAATTAAGGACTGAAATGTGATAAGTGCTACAACTGCGGTGTCT110.10540436949022614No Hit
ACATTCAGTTACACATGTCATGACAGGCCCCATTCCTTTGTCTGTCGTTT110.10540436949022614No Hit
TAGAAAACTCAATTCCTGGCAGGCCCCTAGAGGTGAGGTTCAGAGTGTAC110.10540436949022614No Hit
GTTTGTCTGGAGACACTCTTGGCATCTAGTGGGTGGAGGCCAGGATGCTG110.10540436949022614No Hit
AATGTAGAAGACAGTTTTAAAAAATGTGTTTCACCGTGACAGATCAAACA110.10540436949022614No Hit
GTTTAAAACATGAACAGAGGAGACTGACTTATCAGTGATTCTTCCGCGGG110.10540436949022614No Hit
TTTGTTTTTCTGGGAAGAAGGTCCATAGCTTTCATCAGCACCTTTAAGGG110.10540436949022614No Hit
AAATCAGACCCTTTCATTTAAAAAAAAAAGAGCCTTCGGGGTTGCTTGGT110.10540436949022614No Hit
TATTATATTGTATGCAACACTACCCATCATGGCCAAAAGAGGGAGAAAAT110.10540436949022614No Hit
ATTGGTTTATGGAGAAATGGGAGGGTAAGGTTTGCCTCCTCATATCCCAT110.10540436949022614No Hit
AAAACAAAAGGAACCGACAGGAGTTAAGAGAGGGAAACGCGTTTCCTTTT110.10540436949022614No Hit
TTCTTGTTCTGGACACTGTGCCACACCTTACTCTAAAGAGTAACCTCTAA110.10540436949022614No Hit
CTTTCATCCATCAATAGAAGGGCTGGGCCAGCTGAATGAAAAAGCCCTTC110.10540436949022614No Hit
TCTTAAGTACTATCCTAGTATTCTATCTCCCTCTGTTGGTTGAAGGAGTA110.10540436949022614No Hit
AGACTGGCACTCTTGTAAAACATCAATTGGTGGCTGGGCACAGTGGCTCA110.10540436949022614No Hit
GTCTCTGGCAGAGGCCTGAGCCTCCTGGGATGGCTGGATCTGTCCAGCTC110.10540436949022614No Hit
ATGTATTAATTAGAAATTTAAAGTCTTTCTCTGAAGTAGTTTTCCTGTGG110.10540436949022614No Hit
TTTATATTTAAATCTAAAACCTCTACCGTCCAAAGCCATGGATAACTAGC110.10540436949022614No Hit
TATAGACATACCAGACAAGGGCTGGCCACCACACCGGTCCCAGAGCTGAC110.10540436949022614No Hit
ACTATAGAAATCAGCTTTGACCAAAATGAGAATCACACTGTTTGTATTAA110.10540436949022614No Hit
TAGTTCAGAGACTAGTAGGAGGGTGTCTAAGGGCTCTGCCACCTCCAGGA110.10540436949022614No Hit
ATCCCTCTCAGCCTCTTGGGTTCAGTTTTCTAGAGAGGAAACCACATATG110.10540436949022614No Hit
ATATGACATCGTGTGGCCATCTGAAGACGTCTCTAGAAATTGACAACTGT110.10540436949022614No Hit
GATGGCAGCTTCAGCTCAGATCTCATCTCAAACATCAAGAGCCTTGACTC110.10540436949022614No Hit
AATTCCATTAATTTATCTGATTTTCCCTGTTATCTATTGGACATCTGTAG110.10540436949022614No Hit
TTTTAAAGAACAGTAAGAAGTATTTTTAAATCTTTTAAAAATAAGCAGAA110.10540436949022614No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)