FastQCFastQC Report
Mon 8 Aug 2022
EGAF00006415010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006415010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35398844
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA773300021.84534613616196No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG411700511.630337420058122No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT27335797.722226748421503No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC20511615.794429332212092No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1322450.37358564590414306No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG806020.22769670105611356No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT538360.15208406240610567No Hit
CTCACACACACACACACACACACACACACACACACACACACACACACACA460020.1299533962182494No Hit
CACACACACACACACACACACACACACACACACACACACACACACACAAA435860.12312831458564015No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAG423550.11965079989617741No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)