FastQCFastQC Report
Sun 19 May 2024
EGAF00006419411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006419411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125762767
Total Bases12.7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13351631.061652054777071No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT3396000.27003222662872867TruSeq Adapter, Index 8 (97% over 36bp)
CGAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1723270.13702545205609226No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1501110.11936044632351321No Hit
GAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1409660.1120888187837025No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)