FastQCFastQC Report
Thu 23 May 2024
EGAF00006419451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006419451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90106919
Total Bases9.1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16021511.7780554676383953No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT3049200.33839798695147927TruSeq Adapter, Index 19 (97% over 38bp)
CGAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2545070.2824500080842849No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2308230.2561656780207966No Hit
GAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1893620.210152563312036No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1542380.17117220487807377No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)