FastQCFastQC Report
Sat 25 May 2024
EGAF00006419489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006419489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93992260
Total Bases9.4 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25245172.685877539278234No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT1636120.17406965211816378TruSeq Adapter, Index 6 (97% over 36bp)
CGAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1622310.17260038220168342No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1402590.14922398929443764No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA989130.10523526086084109No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)